Measuring radioactive methane with the liquid scintillation counter.

Applied and environmental microbiology ·Vol. 37 ·No. 5 ·1979-05-00 ·Pages 897-9

Zehnder AJ, Huser B, Brock TD

Abstract

Although a gas proportional counter is the most convenient method of measuring the radioactivity of fixed gases such as methane, it cannot be used when high nonradioactive concentrations of methane are present in the gas phase, due to quenching. If only methane and carbon dioxide are present in radioactive form in the gas phase, a liquid scintillation method for measuring these substances can be used. The procedure is described in detail, and the solubility of methane in liquid scintillation cocktails is determined.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Zehnder A J

Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706.

Huser B

Brock T D

References (4)

4 references, click to expand

Methane formation and methane oxidation by methanogenic bacteria.
J Bacteriol. 1979 Jan;137(1):420-32 PMID: 762019
Rapid method for the radioisotopic analysis of gaseous end products of anaerobic metabolism.
Appl Microbiol. 1974 Aug;28(2):258-61 PMID: 4854029
A new coenzyme of methyl transfer, coenzyme M.
Biochemistry. 1971 Jun 8;10(12):2317-24 PMID: 4398893
Anaerobic degradation of benzoate to methane by a microbial consortium.
Arch Microbiol. 1976 Feb;107(1):33-40 PMID: 1252087

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.37.5.897-899.1979

Published

1979-05-00

Pages

897-9

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC243321

External Links

PubMed source DOI: 10.1128/aem.37.5.897-899.1979 PMC Full Text View journal details

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