Chemically Defined Minimal Medium for Growth of the Anaerobic Cellulolytic Thermophile Clostridium thermocellum.

Applied and environmental microbiology ·Vol. 41 ·No. 4 ·1981-04-00 ·Pages 1060-2

Johnson EA, Madia A, Demain AL

Abstract

A minimal chemically defined medium has been developed for Clostridium thermocellum. The growth factors required are biotin, pyridoxamine, vitamin B(12), and p-aminobenzoic acid.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Johnson E A

Fermentation Microbiology Laboratories, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.

Madia A

Demain A L

References (5)

5 references, click to expand

Cellulolytic and physiological properties of Clostridium thermocellum.
Arch Microbiol. 1977 Jul 26;114(1):1-7 PMID: 20860
A continuous spectrophotometric assay for the determination of cellulase solubilizing activity.
Anal Biochem. 1980 Mar 15;103(1):42-50 PMID: 7377544
Chemically Defined Medium for Growth of Clostridium thermocellum, a Cellulolytic Thermophilic Anaerobe.
Appl Microbiol. 1971 May;21(5):967 PMID: 16349910
Mutants of Escherichia coli requiring methionine or vitamin B12.
J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
Aromatic biosynthesis. III. Role of p-aminobenzoic acid in the formation of vitamin B12.
J Bacteriol. 1951 Aug;62(2):221-30 PMID: 14861182

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.41.4.1060-1062.1981

Published

1981-04-00

Pages

1060-2

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC243859

External Links

PubMed source DOI: 10.1128/aem.41.4.1060-1062.1981 PMC Full Text View journal details

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Chemically Defined Minimal Medium for Growth of the Anaerobic Cellulolytic Thermophile Clostridium thermocellum.

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