Protection of sediment-adsorbed transforming DNA against enzymatic inactivation.

Applied and environmental microbiology ·Vol. 46 ·No. 2 ·1983-08-00 ·Pages 417-20

Aardema BW, Lorenz MG, Krumbein WE

Abstract

The action of DNase I on transforming DNA, both adsorbed to marine sediment and in solution, was investigated. DNase I reduced the transformation frequencies of free DNA more than of adsorbed DNA. Changes in salt concentration or pH did not have a significant influence on the DNA-sediment complex. Soil components other than organic materials and clay minerals can bind DNA and retard its enzymatic degradation.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Aardema B W

Geomicrobiology Division, University of Oldenburg, D-2900 Oldenburg, West Germany.

Lorenz M G

Krumbein W E

References (5)

5 references, click to expand

Ultraviolet inactivation and excision-repair in Bacillus subtilis. I. Construction and characterization of a transformable eightfold auxotrophic strain and two ultraviolet-sensitive derivatives.
Mutat Res. 1972 May;15(1):1-10 PMID: 4623569
Appearance of genetic transforming activity in pneumococcal cultures.
Science. 1960 Oct 28;132(3435):1257-8 PMID: 13731684
Genetic transformation of Bacillus subtilis by extracellular DNA.
Biochem Biophys Res Commun. 1962 Jun 4;7:467-70 PMID: 13919045
Genetic exchange in Bacillus subtilis in soil.
Mol Gen Genet. 1978 Nov 9;166(3):287-90 PMID: 105243
Adsorption of ribonucleic acid on Bentonite.
Anal Biochem. 1969 Oct 15;32(1):150-4 PMID: 4921406

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.46.2.417-420.1983

Published

1983-08-00

Pages

417-20

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC239404

External Links

PubMed source DOI: 10.1128/aem.46.2.417-420.1983 PMC Full Text View journal details

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Protection of sediment-adsorbed transforming DNA against enzymatic inactivation.

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