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PMID: 16347370 Published · ppublish English Journal Article

Production of Pili (Fimbriae) by Pseudomonas fluorescens and Correlation with Attachment to Corn Roots.

Applied and environmental microbiology ·Vol. 53 ·No. 7 ·1987-07-00 ·Pages 1397-405

Vesper SJ

Abstract

Pseudomonas fluorescens isolates 13525 and 2-79 were grown in Luria broth and low-nutrient medium (LNM). Pililike fibrils were very rarely produced in Luria broth but were abundantly produced in LNM. In LNM the pili were peritrichously distributed and had diameters ranging from 3 to 8 nm. Pili were purified from strain 2-79, and the pilin subunit was found to have a molecular weight of about 34,000. Strain 2-79 produced two colony types on Luria agar, nonmucoidal and mucoidal. Cells in LNM cultures of the nonmucoidal colony type were highly piliated, and cells from the mucoidal type were nearly devoid of pili. The presence of pili on nonmucoidal isolate 2-79 was quantitatively correlated with hydrophobic attachment to polystyrene, hemagglutination, and attachment to corn roots.

Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Vesper S J
Battelle-Kettering Laboratory, Yellow Springs, Ohio 45387.
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.53.7.1397-1405.1987
Published
1987-07-00
Pages
1397-405
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC203883
External Links
PubMed source DOI: 10.1128/aem.53.7.1397-1405.1987 PMC Full Text View journal details
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