Genetic Diversity among Rhizobium leguminosarum bv. Trifolii Strains Revealed by Allozyme and Restriction Fragment Length Polymorphism Analyses.

Applied and environmental microbiology ·Vol. 57 ·No. 12 ·1991-12-00 ·Pages 3489-95

Demezas DH, Reardon TB, Watson JM, Gibson AH

Abstract

Allozyme electrophoresis and restriction fragment length polymorphism (RFLP) analyses were used to examine the genetic diversity of a collection of 18 Rhizobium leguminosarum bv. trifolii, 1 R. leguminosarum bv. viciae, and 2 R. meliloti strains. Allozyme analysis at 28 loci revealed 16 electrophoretic types. The mean genetic distance between electrophoretic types of R. leguminosarum and R. meliloti was 0.83. Within R. leguminosarum, the single strain of bv. viciae differed at an average of 0.65 from strains of bv. trifolii, while electrophoretic types of bv. trifolii differed at a range of 0.23 to 0.62. Analysis of RFLPs around two chromosomal DNA probes also delineated 16 unique RFLP patterns and yielded genetic diversity similar to that revealed by the allozyme data. Analysis of RFLPs around three Sym (symbiotic) plasmid-derived probes demonstrated that the Sym plasmids reflect genetic divergence similar to that of their bacterial hosts. The large genetic distances between many strains precluded reliable estimates of their genetic relationships.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Demezas D H

Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, Canberra City, ACT 2601, and Evolutionary Biology Unit, South Australian Museum, Adelaide, South Australia 5000, Australia.

Reardon T B

Watson J M

Gibson A H

References (23)

23 references, click to expand

Genetic structure of natural populations of the nitrogen-fixing bacterium Rhizobium meliloti.
Appl Environ Microbiol. 1990 Jan;56(1):187-94 PMID: 1689982
Rhizobium Population Genetics: Enzyme Polymorphism in Rhizobium leguminosarum from Plants and Soil in a Pea Crop.
Appl Environ Microbiol. 1987 Feb;53(2):397-402 PMID: 16347287
Incompatibility group P plasmids: genetics, evolution, and use in genetic manipulation.
Annu Rev Microbiol. 1987;41:77-101 PMID: 3318684
Paleoepidemiologic investigation of Legionnaires disease at Wadsworth Veterans Administration Hospital by using three typing methods for comparison of legionellae from clinical and environmental sources.
J Clin Microbiol. 1986 Jun;23(6):1121-6 PMID: 3711303
Multiple forms of D (-)3-hydroxybutyrate dehydrogenase in Rhizobium.
J Gen Microbiol. 1969 Aug;57(3):287-92 PMID: 4312036
Properties of an R factor from Pseudomonas aeruginosa.
J Bacteriol. 1971 Dec;108(3):1244-9 PMID: 4945193
Construction of a genetic linkage map in man using restriction fragment length polymorphisms.
Am J Hum Genet. 1980 May;32(3):314-31 PMID: 6247908
The human pro-opiomelanocortin gene: organization, sequence, and interspersion with repetitive DNA.
DNA. 1982;1(2):133-43 PMID: 6299668
Genetic diversity and structure in Escherichia coli populations.
Science. 1980 Oct 31;210(4469):545-7 PMID: 6999623
Fingerprinting bacterial chromosomal DNA with restriction endonuclease EcoRI: comparison of Rhizobium spp. and identification of mutants.
Can J Microbiol. 1979 Jul;25(7):803-7 PMID: 476554
Evidence for genetic exchange and recombination of Rhizobium symbiotic plasmids in a soil population.
Appl Environ Microbiol. 1987 Dec;53(12):2942-7 PMID: 16347509
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Complementary methodologies to identify specific agrobacterium strains.
Appl Environ Microbiol. 1987 Nov;53(11):2660-5 PMID: 16347485
Diversity and Dynamics of Indigenous Rhizobium japonicum Populations.
Appl Environ Microbiol. 1980 Nov;40(5):931-8 PMID: 16345658
Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics.
Appl Environ Microbiol. 1986 May;51(5):873-84 PMID: 2425735
Genetic diversity and relationships among isolates of Rhizobium leguminosarum biovar phaseoli.
Appl Environ Microbiol. 1988 Nov;54(11):2825-32 PMID: 3214160
Genetic structure of populations of Legionella pneumophila.
J Bacteriol. 1985 Sep;163(3):1021-37 PMID: 4030689
R factor transfer in Rhizobium leguminosarum.
J Gen Microbiol. 1974 Sep;84(1):188-98 PMID: 4612098
Determination of multiple forms of esterases in rhizobium by paper electrophoresis.
J Gen Microbiol. 1970 Apr;61(1):121-9 PMID: 5489059
Evidence for clonal population structure in Escherichia coli.
Proc Natl Acad Sci U S A. 1984 Jan;81(1):198-201 PMID: 6364134
Genetic diversity and temporal variation in the E. coli population of a human host.
Genetics. 1981 Jul;98(3):467-90 PMID: 7037535
Influence of Location, Host Cultivar, and Inoculation on the Composition of Naturalized Populations of Rhizobium meliloti in Medicago sativa Nodules.
Appl Environ Microbiol. 1986 May;51(5):1077-84 PMID: 16347054
Genetic diversity and relationships of two pathovars of Pseudomonas syringae.
J Gen Microbiol. 1988 Jul;134(7):1949-60 PMID: 2907755

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.57.12.3489-3495.1991

Published

1991-12-00

Pages

3489-95

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC184001

External Links

PubMed source DOI: 10.1128/aem.57.12.3489-3495.1991 PMC Full Text View journal details

Analysis Services

Genetic Diversity among Rhizobium leguminosarum bv. Trifolii Strains Revealed by Allozyme and Restriction Fragment Length Polymorphism Analyses.

Contact

WeChat Official Account