Separation of methanotrophic bacteria by using percoll and its application to isolation of mixed and pure cultures.

Applied and environmental microbiology ·Vol. 57 ·No. 12 ·1991-12-00 ·Pages 3656-9

Putzer KP, Buchholz LA, Lidstrom ME, Remsen CC

Abstract

Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Putzer K P

Center for Great Lakes Studies and Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53201, and Keck Laboratories, 138-78 California Institute of Technology, Pasadena, California 91125.

Buchholz L A

Lidstrom M E

Remsen C C

References (9)

9 references, click to expand

Specific binding of rat liver plasma membranes by rat liver cells.
Biochem Biophys Res Commun. 1977 Jul 25;77(2):665-70 PMID: 901496
Isolation and characterization of marine methanotrophs.
Antonie Van Leeuwenhoek. 1988;54(3):189-99 PMID: 3138946
A survey of buoyant density of microorganisms in pure cultures and natural samples.
Microbiologia. 1985 Sep;1(1-2):53-65 PMID: 3917196
Enrichment, isolation and some properties of methane-utilizing bacteria.
J Gen Microbiol. 1970 May;61(2):205-18 PMID: 5476891
Separation of Syntrophomonas wolfei from Methanospirillum hungatii in Syntrophic Cocultures by Using Percoll Gradients.
Appl Environ Microbiol. 1987 May;53(5):1183-5 PMID: 16347346
Discontinuous density gradient separation of human mononuclear leucocytes using Percoll as gradient medium.
J Immunol Methods. 1979;30(1):1-10 PMID: 41875
Use of oligodeoxynucleotide signature probes for identification of physiological groups of methylotrophic bacteria.
Appl Environ Microbiol. 1990 Sep;56(9):2858-65 PMID: 1967116
Harvest of planktonic marine algae by centrifugation into gradients of silica in the CF-6 continuous-flow zonal rotor.
Biol Bull. 1974 Aug;147(1):136-45 PMID: 4845246
A rapid single centrifugation step method for the separation of erythrocytes, granulocytes and mononuclear cells on continuous density gradients of Percoll.
J Immunol Methods. 1980;32(3):209-14 PMID: 7351486

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.57.12.3656-3659.1991

Published

1991-12-00

Pages

3656-9

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC184030

External Links

PubMed source DOI: 10.1128/aem.57.12.3656-3659.1991 PMC Full Text View journal details

Analysis Services

Separation of methanotrophic bacteria by using percoll and its application to isolation of mixed and pure cultures.

Contact

WeChat Official Account