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PMID: 16374505 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast Trf5p is a nuclear poly(A) polymerase.

EMBO reports ·Vol. 7 ·No. 2 ·2006-02-00 ·Pages 205-11

Houseley J, Tollervey D

Abstract

Recent analyses have shown that the activity of the yeast nuclear exosome is stimulated by the Trf4p-Air1/2p-Mtr4p polyadenylation (TRAMP) complex. Here, we report that strains lacking the Rrp6p component of the nuclear exosome accumulate polyadenylated forms of many different ribosomal RNA precursors (pre-rRNAs). This polyadenylation is reduced in strains lacking either the poly(A) polymerase Trf4p or its close homologue Trf5p. In contrast, polyadenylation is enhanced by overexpression of Trf5p. Polyadenylation is also markedly increased in strains lacking the RNA helicase Mtr4p, indicating that it is required to couple poly(A) polymerase activity to degradation. Tandem affinity purification-tagged purified Trf5p showed polyadenylation activity in vitro, which was abolished by a double point mutation in the predicted catalytic site. Trf5p co-purified with Mtr4p and Air1p, indicating that it forms a complex, designated TRAMP5, that has functions that partially overlap with the TRAMP complex.

MeSH Terms
Amino Acid Sequence Cell Nucleus/metabolism DNA-Directed RNA Polymerases/chemistry,genetics,metabolism Genes, Fungal In Vitro Techniques Molecular Sequence Data Point Mutation Polyadenylation Polynucleotide Adenylyltransferase/metabolism RNA Helicases/metabolism RNA, Fungal/genetics,metabolism RNA, Messenger/metabolism Saccharomyces cerevisiae/enzymology,genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/chemistry,genetics,metabolism
Chemicals
RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Proteins Polynucleotide Adenylyltransferase DNA-Directed RNA Polymerases Trf5 protein, S cerevisiae RNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Houseley Jonathan
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Swann Building, King's Buildings, Edinburgh EH9 3JR, UK.
Tollervey David
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1038/sj.embor.7400612
Published
2006-02-00
Pages
205-11
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1369253
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1038/sj.embor.7400612 PMC Full Text View journal details
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