Home LiteratureArticle Details

PMID: 16375848 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Acute effect of fatty acids on metabolism and mitochondrial coupling in skeletal muscle.

Biochimica et biophysica acta ·Vol. 1757 ·No. 1 ·2006-01-00 ·Pages 57-66

Hirabara SM, Silveira LR, Alberici LC, Leandro CV, Lambertucci RH, Polimeno GC, Cury Boaventura MF, Procopio J, Vercesi AE, Curi R

Abstract

Acute effects of free fatty acids (FFA) were investigated on: (1) glucose oxidation, and UCP-2 and -3 mRNA and protein levels in 1 h incubated rat soleus and extensor digitorium longus (EDL) muscles, (2) mitochondrial membrane potential in cultured skeletal muscle cells, (3) respiratory activity and transmembrane electrical potential in mitochondria isolated from rat skeletal muscle, and (4) oxygen consumption by anesthetized rats. Long-chain FFA increased both basal and insulin-stimulated glucose oxidation in incubated rat soleus and EDL muscles and reduced mitochondrial membrane potential in C2C12 myotubes and rat skeletal muscle cells. Caprylic, palmitic, oleic, and linoleic acid increased O(2) consumption and decreased electrical membrane potential in isolated mitochondria from rat skeletal muscles. FFA did not alter UCP-2 and -3 mRNA and protein levels in rat soleus and EDL muscles. Palmitic acid increased oxygen consumption by anesthetized rats. These results suggest that long-chain FFA acutely lead to mitochondrial uncoupling in skeletal muscle.

MeSH Terms

Animals Carrier Proteins/genetics,metabolism Cells, Cultured Coenzyme A/metabolism Fatty Acids/pharmacology Glucose/metabolism In Vitro Techniques Insulin/pharmacology Ion Channels Linoleic Acid/pharmacology Membrane Potentials/drug effects Membrane Transport Proteins/genetics,metabolism Mitochondria, Muscle/drug effects,metabolism Mitochondrial Membranes/drug effects Mitochondrial Proteins/genetics,metabolism Muscle Cells/drug effects Muscle Fibers, Skeletal/drug effects,metabolism Muscle, Skeletal/cytology,drug effects,metabolism Oleic Acid/pharmacology Oxygen Consumption/drug effects Palmitic Acid/pharmacology RNA, Messenger/analysis,metabolism Rats Uncoupling Protein 2 Uncoupling Protein 3

Chemicals

Carrier Proteins Fatty Acids Insulin Ion Channels Membrane Transport Proteins Mitochondrial Proteins RNA, Messenger Ucp2 protein, rat Uncoupling Protein 2 Uncoupling Protein 3 Oleic Acid Palmitic Acid Linoleic Acid Glucose Coenzyme A

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Hirabara Sandro M

Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Cidade Universitária, Av. Professor Lineu Prestes, 1524 Butantã, São Paulo, SP 05508-900, Brazil.

Silveira Leonardo R

Alberici Luciane C

Leandro Carol V G

Lambertucci Rafael H

Polimeno Gisele C

Cury Boaventura Maria F

Procopio Joaquim

Vercesi Anibal E

Curi Rui

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/j.bbabio.2005.11.007

Published

2006-01-00

Epub

2005-00-13

Pages

57-66

Language

English

Region

Netherlands

NLM ID

0217513

Subset

External Links

PubMed source DOI: 10.1016/j.bbabio.2005.11.007

Analysis Services

Acute effect of fatty acids on metabolism and mitochondrial coupling in skeletal muscle.

Contact

WeChat Official Account