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PMID: 163913 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mosquito cells infected with vesicular stomatitis virus yield unsialylated virions of low infectivity.

Journal of virology ·Vol. 15 ·No. 4 ·1975-04-00 ·Pages 1029-32

Schloemer RH, Wagner RR

Abstract

Vesicular stomatitis virus propagated in and released from Aedes albopictus cells had the normal complement of viral proteins; the glycoprotein contained carbohydrate but no sialic acid. These virions had markedly reduced hemagglutinating activity and exhibited a very high ratio of physical particles to infectious virus. In vitro sialylation of vesicular stomatitis virions grown in mosquito cells resulted in a 100-fold increase in both infectivity and hemagglutination titers to levels approaching those of virus grown in BHK-21 cells. These experiments provide an example of host-controlled modification of viral infectivity.

MeSH Terms
Aedes/microbiology Animals Cell Line Cricetinae Glucosamine Glycoproteins/analysis Hemagglutination, Viral Kidney Sialic Acids/analysis Tritium Vesicular stomatitis Indiana virus/analysis,growth & development Viral Proteins/analysis Virus Replication
Chemicals
Glycoproteins Sialic Acids Viral Proteins Tritium Glucosamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schloemer R H
Wagner R R
References (12)
12 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.4.1029-1032.1975
Published
1975-04-00
Pages
1029-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354549
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.15.4.1029-1032.1975 PMC Full Text View journal details
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