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PMID: 164029 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Identification of a calcium-binding protein as a calcium-dependent regulator of brain adenylate cyclase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 1 ·1975-01-00 ·Pages 64-8

Brostrom CO, Huang YC, Breckenridge BM, Wolff DJ

Abstract

An activating factor of adenylate cyclase (EC 4.6.1.1) HAS BEEN OBTAINED FROM DETERGENT-DISPERSED PREPARATIONS OF PORCINE CEREBRAL CORTEX BY COLUMN CHROMATOGRAPHY ON ECTEOLA-cellulose. The factor was identified by acrylamide gel electrophoresis and by enzyme activation studies as the Ca2+-binding protein that regulates the activity of a brain cyclic nucleotide phosphodiesterase. This Ca2+-binding protein confers a Ca2+-dependent activation upon the adenylate cyclase, which is reversed by the subsequent addition of egta in excess of the free Ca2+. It is proposed that this Ca2+-dependent regulator controls enzymatic activities responsible for the synthesis of adenosine 3':5'-monophosphate and for the hydrolysis of guanosine 3':5'-monophosphate.

MeSH Terms
Adenylyl Cyclases/metabolism Animals Brain/enzymology Calcium/metabolism,pharmacology Cerebral Cortex/analysis Cyclic AMP/biosynthesis Cyclic GMP/metabolism Electrophoresis, Polyacrylamide Gel Enzyme Activation/drug effects Ethylene Glycols/pharmacology Nerve Tissue Proteins/isolation & purification,metabolism,pharmacology Phosphoric Diester Hydrolases/metabolism Protein Binding Swine
Chemicals
Ethylene Glycols Nerve Tissue Proteins Cyclic AMP Phosphoric Diester Hydrolases Adenylyl Cyclases Cyclic GMP Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brostrom C O
Huang Y C
Breckenridge B M
Wolff D J
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.1.64
Published
1975-01-00
Pages
64-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432241
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.1.64 PMC Full Text View journal details
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