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PMID: 16437602 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oral vaccination of mice against rodent malaria with recombinant Lactococcus lactis expressing MSP-1(19).

World journal of gastroenterology ·Vol. 11 ·No. 44 ·2005-11-28 ·Pages 6975-80

Zhang ZH, Jiang PH, Li NJ, Shi M, Huang W

Abstract

To construct the recombinant Lactococcus lactis as oral delivery vaccination against malaria. The C-terminal 19-ku fragments of MSP1 (MSP-1(19)) of Plasmodium yoelii 265-BY was expressed in L. lactis and the recombinant L. lactis was administered orally to BALB/c and C57BL/6 mice. After seven interval vaccinations within 4 wk, the mice were challenged with P. yoelii 265-BY parasites of erythrocytic stage. The protective efficacy of recombinant L. lactis was evaluated. The peak parasitemias in average for the experiment groups of BALB/c and C57BL/6 mice were 0.8+/-0.4% and 20.8+/-26.5%, respectively, and those of their control groups were 12.0+/-0.8% and 60.8+/-9.6%, respectively. None of the BALB/c mice in both experimental group and control group died during the experiment. However, all the C57BL/6 mice in the control group died within 23 d and all the vaccinated mice survived well. The results imply the potential of recombinant L. lactis as oral delivery vaccination against malaria.

MeSH Terms
Administration, Oral Animals Humans Lactococcus lactis/immunology Malaria/immunology,prevention & control,veterinary Malaria Vaccines/administration & dosage,therapeutic use Merozoite Surface Protein 1/genetics,metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Parasitemia Peptide Fragments/genetics,metabolism Plasmodium yoelii/immunology Protein Subunits/genetics,metabolism Protozoan Proteins/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Rodent Diseases/immunology,prevention & control Survival Rate Vaccination
Chemicals
Malaria Vaccines Merozoite Surface Protein 1 Peptide Fragments Protein Subunits Protozoan Proteins Recombinant Fusion Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zhang Zhi-Hong
Department of Biochemistry, Fudan University, 220 Han Dan Road, Shanghai 200433, China.
Jiang Pei-Hong
Li Ning-Jun
Shi Mi
Huang Weida
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Article Info
Journal
World journal of gastroenterology
Abbr.
World J Gastroenterol
ISSN
1007-9327
DOI
10.3748/wjg.v11.i44.6975
Published
2005-11-28
Pages
6975-80
Language
English
Region
United States
NLM ID
100883448
PMCID
PMC4717040
Subset
IM
External Links
PubMed source DOI: 10.3748/wjg.v11.i44.6975 PMC Full Text View journal details
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