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PMID: 16449570 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Target hub proteins serve as master regulators of development in yeast.

Genes & development ·Vol. 20 ·No. 4 ·2006-02-15 ·Pages 435-48

Borneman AR, Leigh-Bell JA, Yu H, Bertone P, Gerstein M, Snyder M

Abstract

To understand the organization of the transcriptional networks that govern cell differentiation, we have investigated the transcriptional circuitry controlling pseudohyphal development in Saccharomyces cerevisiae. The binding targets of Ste12, Tec1, Sok2, Phd1, Mga1, and Flo8 were globally mapped across the yeast genome. The factors and their targets form a complex binding network, containing patterns characteristic of autoregulation, feedback and feed-forward loops, and cross-talk. Combinatorial binding to intergenic regions was commonly observed, which allowed for the identification of a novel binding association between Mga1 and Flo8, in which Mga1 requires Flo8 for binding to promoter regions. Further analysis of the network showed that the promoters of MGA1 and PHD1 were bound by all of the factors used in this study, identifying them as key target hubs. Overexpression of either of these two proteins specifically induced pseudohyphal growth under noninducing conditions, highlighting them as master regulators of the system. Our results indicate that target hubs can serve as master regulators whose activity is sufficient for the induction of complex developmental responses and therefore represent important regulatory nodes in biological networks.

MeSH Terms
Blotting, Western Cell Differentiation/physiology Chromatin Immunoprecipitation DNA-Binding Proteins/metabolism Models, Biological Nuclear Proteins/metabolism Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Protein Binding Repressor Proteins/metabolism Saccharomyces cerevisiae/cytology,genetics,growth & development Saccharomyces cerevisiae Proteins/metabolism Trans-Activators/metabolism Transcription Factors/metabolism Transcription, Genetic/physiology
Chemicals
DNA-Binding Proteins FLO8 protein, S cerevisaie Nuclear Proteins PHD1 protein, S cerevisiae Repressor Proteins SOK2 protein, S cerevisiae STE12 protein, S cerevisiae Saccharomyces cerevisiae Proteins TEC1 protein, S cerevisiae Trans-Activators Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Borneman Anthony R
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06511, USA.
Leigh-Bell Justine A
Yu Haiyuan
Bertone Paul
Gerstein Mark
Snyder Michael
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1389306
Published
2006-02-15
Epub
2006-00-31
Pages
435-48
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1369046
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.1389306 PMC Full Text View journal details
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