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PMID: 16452502 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Selective and antagonistic functions of SWI/SNF and Mi-2beta nucleosome remodeling complexes during an inflammatory response.

Genes & development ·Vol. 20 ·No. 3 ·2006-02-01 ·Pages 282-96

Ramirez-Carrozzi VR, Nazarian AA, Li CC, Gore SL, Sridharan R, Imbalzano AN, Smale ST

Abstract

Studies of mammalian genes activated in response to an acute stimulus have suggested diverse mechanisms through which chromatin structure and nucleosome remodeling events contribute to inducible gene transcription. However, because of this diversity, the logical organization of the genome with respect to nucleosome remodeling and gene induction has remained obscure. Numerous proinflammatory genes are rapidly induced in macrophages in response to microbial infection. Here, we show that in lipopolysaccharide-stimulated macrophages, the catalytic BRG1/BRM subunits of the SWI/SNF class of ATP-dependent nucleosome remodeling complexes are consistently required for the activation of secondary response genes and primary response genes induced with delayed kinetics, but not for rapidly induced primary response genes. Surprisingly, a Mi-2beta complex was selectively recruited along with the SWI/SNF complexes to the control regions of secondary response and delayed primary response genes, with the Mi-2beta complex acting antagonistically to limit the induction of these gene classes. SWI/SNF and Mi-2beta complexes influenced cell size in a similarly antagonistic manner. These results provide insight into the differential contributions of nucleosome remodeling complexes to the rapid induction of defined classes of mammalian genes and reveal a robust anti-inflammatory function of Mi-2beta.

MeSH Terms
Adenosine Triphosphatases/physiology Animals Cells, Cultured Chromosomal Proteins, Non-Histone/antagonists & inhibitors,physiology DNA Helicases Gene Expression Regulation/drug effects,genetics,physiology Inflammation/metabolism Kinetics Lipopolysaccharides Macrophages/metabolism Mice Microscopy, Fluorescence Models, Biological Nuclear Proteins/metabolism Nucleosomes/metabolism Promoter Regions, Genetic Signal Transduction Transcription Factors/antagonists & inhibitors,metabolism,physiology Transcriptional Activation
Chemicals
Chromosomal Proteins, Non-Histone Lipopolysaccharides Nuclear Proteins Nucleosomes SWI-SNF-B chromatin-remodeling complex Transcription Factors Adenosine Triphosphatases Smarca4 protein, mouse Mi-2beta protein, mouse DNA Helicases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ramirez-Carrozzi Vladimir R
Howard Hughes Medical Institute and Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California 90095-1662, USA.
Nazarian Aaron A
Li Caiyi C
Gore Sarah L
Sridharan Rupa
Imbalzano Anthony N
Smale Stephen T
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1383206
Published
2006-02-01
Pages
282-96
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1361700
Subset
IM
Grants
NCI NIH HHS · T32 CA009120 · United States
NCI NIH HHS · CA009120 · United States
External Links
PubMed source DOI: 10.1101/gad.1383206 PMC Full Text View journal details
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