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PMID: 16453431 Published · ppublish English Journal Article

A cAMP-dependent protein kinase is present in differentiating Dictyostelium discoideum cells.

The EMBO journal ·Vol. 1 ·No. 9 ·1982-00-00 ·Pages 1063-8

Gunzburg J, Veron M

Abstract

We demonstrate the occurrence of a cAMP-dependent protein kinase in Dictyostelium discoideum cells at the terminal stage of differentiation. A cAMP-binding component was purified to homogeneity by affinity chromatography. This subunit inhibits the activity of purified catalytic subunit from beef heart protein kinase; the inhibition is reversed upon addition of cAMP. The protein is highly specific for cAMP and has a dissociation constant of 4 nM. The isolated regulatory subunit is a monomer of 39 K, with a sedimentation coefficient of 3.5S and a frictional coefficient of 1.24. The differences between this regulatory subunit and regulatory subunits of protein kinases from other sources are discussed.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gunzburg J
Unité de Biochimie Cellulaire, Départment de Biochimie et Génétique Moléculaire, Institut Pasteur, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France.
Veron M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1063-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553163
External Links
PubMed source PMC Full Text View journal details
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