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PMID: 16453461 Published · ppublish English Journal Article

Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene.

The EMBO journal ·Vol. 2 ·No. 6 ·1983-00-00 ·Pages 947-52

Downie JA, Ma QS, Knight CD, Hombrecher G, Johnston AW

Abstract

The region of the Rhizobium leguminosarum plasmid pRL1JI involved in nodulation and nitrogen fixation has been cloned on a series of four overlapping cosmid clones. These clones represent 60 kb of pRL1JI DNA on which a series of Tn5-induced fix and nod alleles have been identified, with the two most distant alleles being separated by 45 kb of DNA. The mutant alleles fell into three groups, two clusters of fix alleles separated by one cluster of nod alleles. Within one group of fix alleles, DNA homologous to the nifA gene of Klebsiella pneumoniae has been identified, whereas the pRL1JI DNA homologous to the K. pneumoniae nitrogenase genes is present within the other group of fix alleles.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Downie J A
John Innes Institute, Colney Lane, Norwich NR4 7UH, UK.
Ma Q S
Knight C D
Hombrecher G
Johnston A W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
947-52
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555213
External Links
PubMed source PMC Full Text View journal details
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