Home LiteratureArticle Details
PMID: 16453607 Published · ppublish English Journal Article

In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi.

The EMBO journal ·Vol. 4 ·No. 3 ·1985-03-00 ·Pages 787-92

Gijsegem F, Toussaint A, Schoonejans E

Abstract

Using an RP4 plasmid which carries a mini-Mu prophage which allows it to integrate spontaneously random pieces of its host chromosome, we cloned in vivo at least some of the pectate lyase and cellulase genes of the Erwinia chrysanthemi strain B374. The RP4-prime plasmids were used to localize the cloned genes on the B374 chromosome by co-transposition mapping and to subclone most of the genes in a classic high copy number plasmid vector.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gijsegem F
Laboratoire de Génétique, Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium.
Toussaint A
Schoonejans E
References (23)
23 references, click to expand
  1. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  2. Bacteriophage Mu-1: a tool to transpose and to localize bacterial genes.
    J Mol Biol. 1976 Jul 5;104(3):525-39 PMID: 781292
  3. Genetic control of prophage insertion specificity in bacteriophages lambda and 21.
    Virology. 1967 Jul;32(3):465-74 PMID: 5339457
  4. Molecular cloning of pectate lyase genes from Erwinia chrysanthemi and their expression in Escherichia coli.
    J Bacteriol. 1984 Sep;159(3):825-31 PMID: 6090392
  5. Isolation of a temperature-sensitive derivative of RP1.
    Plasmid. 1980 May;3(3):343-7 PMID: 6100900
  6. Physical characterization of mini-mu and mini-D108.
    Gene. 1981 Jun-Jul;14(1-2):103-13 PMID: 6266926
  7. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  8. Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase.
    J Bacteriol. 1984 Dec;160(3):1199-203 PMID: 6389513
  9. In vivo cloning of Erwinia carotovora genes involved in the catabolism of hexuronates.
    J Bacteriol. 1983 Jun;154(3):1227-35 PMID: 6853444
  10. [The reciprocal antagonistic properties among the Erwinia. Discussion of the taxonomic position of this genus].
    C R Hebd Seances Acad Sci. 1961 Jul 31;253:913-5 PMID: 13904218
  11. TOPOGRAPHY OF COTRANSDUCIBLE ARGININE MUTATIONS IN ESCHERICHIA COLI K-12.
    Genetics. 1965 Feb;51:167-79 PMID: 14292146
  12. The transposon Tn1 as a probe for studying ColE1 structure and function.
    Mol Gen Genet. 1977 Mar 7;151(2):151-60 PMID: 327263
  13. A restriction endonuclease analysis of the bacterial plasmid controlling the ecoRI restriction and modification of DNA.
    Fed Proc. 1976 Jul;35(9):2037-43 PMID: 776705
  14. Isolation and characterization of Erwinia chrysanthemi mutants defective in degradation of hexuronates.
    J Bacteriol. 1985 Feb;161(2):702-8 PMID: 3968035
  15. Properties of RP4, an R factor which originated in Pseudomonas aeruginosa S8.
    J Bacteriol. 1972 Nov;112(2):690-6 PMID: 4628745
  16. Properties of four mutants of Escherichia coli defective in genetic recombination.
    Mutat Res. 1966 Oct;3(5):381-92 PMID: 5339653
  17. Chromosome transfer and R-prime formation by an RP4::mini-Mu derivative in Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae, and Proteus mirabilis.
    Plasmid. 1982 Jan;7(1):30-44 PMID: 6211681
  18. Utilization of a thermosensitive episome bearing transposon TN10 to isolate Hfr donor strains of Erwinia carotovora subsp. chrysanthemi.
    J Bacteriol. 1982 Apr;150(1):122-31 PMID: 6277860
  19. Detection of depolymerase isoenzymes after electrophoresis or electrofocusing, or in titration curves.
    Anal Biochem. 1984 Jun;139(2):383-9 PMID: 6433742
  20. Genetics of Erwinia species.
    Annu Rev Microbiol. 1980;34:645-76 PMID: 7002034
  21. A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12.
    Genetics. 1964 Feb;49:237-46 PMID: 14124942
  22. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  23. [2-Keto-3-deoxy-gluconate transport system in E. coli K 12: map location of a structural gene and of its operator].
    Mol Gen Genet. 1973 Mar 1;121(2):163-80 PMID: 4571523
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-03-00
Pages
787-92
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554257
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2