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PMID: 16453695 Published · ppublish English Journal Article

Identification of plant promoters in situ by T-DNA-mediated transcriptional fusions to the npt-II gene.

The EMBO journal ·Vol. 5 ·No. 8 ·1986-08-00 ·Pages 1755-60

Teeri TH, Herrera-Estrella L, Depicker A, Van Montagu M, Palva ET

Abstract

We have constructed an Agrobacterium-mediated plant vector,pHTT88,which upon integration into the plant chromosomes can produce transcriptional fusions between upstream control elements in the plant genome and the gene for neomycin phosphotransferase II (npt-II). The vector is based on a juxtaposition of a T-DNA right border to the coding sequence of npt-II. Using this system it is possible to isolate kanamycin-resistant plant calli where the site of integration is suitably positioned downstream of plant transcription signals. We have demonstrated that the NPT-II activity of such tobacco transformants shows different tissue-specific patterns of regulation in the regenerated plants. Among eight transformants analyzed, we have isolated one gene fusion expressed only in roots and others expressed only in stem. The results demonstrate that a gene fusion vector can be used effectively in plants to identify and characterize regulatory elements.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Teeri T H
Molecular Genetics Laboratory, Department of Genetics, University of Helsinki, SF-00100 Helsinki, Finland.
Herrera-Estrella L
Depicker A
Van Montagu M
Palva E T
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-08-00
Pages
1755-60
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167037
External Links
PubMed source PMC Full Text View journal details
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