Home LiteratureArticle Details
PMID: 16453761 Published · ppublish English Journal Article

Functional analysis of sequences required for transcriptional activation of a soybean heat shock gene in transgenic tobacco plants.

The EMBO journal ·Vol. 6 ·No. 5 ·1987-05-00 ·Pages 1161-6

Baumann G, Raschke E, Bevan M, Schöffl F

Abstract

The 5' DNA sequences involved in the thermal inducibility of the soybean heat shock gene hs6871 were analysed in transgenic tobacco plants. The transcriptional activity of various in vitro generated deletion mutants was examined by Northern blot analysis, S1 nuclease mapping and dot-blot hybridization. At least 181 bp upstream from the translational start site are sufficient for thermal induction at 40 degrees C and correct initiation of transcripts. Full promoter activity with the induction of wild-type levels of transcripts requires additional upstream sequences contained within 439 bp 5' to the coding sequence. Our results suggest that faithful regulation and the generation of high levels of hs6871-specific mRNA depend on the presence of sequences which show homology to the 14-bp heat shock consensus element of Drosophila and, in addition, on as yet unidentified enhancer-like upstream sequences.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Baumann G
Universität Bielefeld, Fakultät für Biologie (Genetik), D-4800 Bielefeld 1, FRG.
Raschke E
Bevan M
Schöffl F
References (27)
27 references, click to expand
  1. Genes for low-molecular-weight heat shock proteins of soybeans: sequence analysis of a multigene family.
    Mol Cell Biol. 1985 Dec;5(12):3417-28 PMID: 3939317
  2. Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster.
    Cell. 1986 Aug 15;46(4):521-30 PMID: 3015418
  3. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  4. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  5. The DNA sequence analysis of soybean heat-shock genes and identification of possible regulatory promoter elements.
    EMBO J. 1984 Nov;3(11):2491-7 PMID: 16453563
  6. An analysis of mRNAs for a group of heat shock proteins of soybean using cloned cDNAs.
    J Mol Appl Genet. 1982;1(4):301-14 PMID: 6896722
  7. Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies.
    Cell. 1984 Sep;38(2):391-8 PMID: 6432342
  8. Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium.
    EMBO J. 1985 Aug;4(8):1921-6 PMID: 16453629
  9. Heat shock proteins of higher plants.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3526-30 PMID: 16593032
  10. A simple and general method for transferring genes into plants.
    Science. 1985 Mar 8;227(4691):1229-31 PMID: 17757866
  11. An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extracts.
    Nature. 1985 Sep 5-11;317(6032):84-7 PMID: 2993917
  12. Light regulation of plant gene expression by an upstream enhancer-like element.
    Nature. 1985 Dec 12-18;318(6046):579-82 PMID: 3865055
  13. Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8419-23 PMID: 3909145
  14. Sequences required for in vitro transcriptional activation of a Drosophila hsp 70 gene.
    Cell. 1985 Sep;42(2):527-37 PMID: 4028160
  15. Isolation of poly(A)+ RNA by paper affinity chromatography.
    Anal Biochem. 1984 Sep;141(2):329-36 PMID: 6208812
  16. Integration, transcription, and control of a Drosophila heat shock gene in mouse cells.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):7038-42 PMID: 6273904
  17. Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.
    J Bacteriol. 1982 Jan;149(1):114-22 PMID: 6274841
  18. Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.
    Nature. 1984 May 17-23;309(5965):229-34 PMID: 6325944
  19. A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.
    Cell. 1984 May;37(1):273-83 PMID: 6722872
  20. A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene.
    Cell. 1982 Sep;30(2):517-28 PMID: 6814763
  21. Expression of foreign genes in regenerated plants and in their progeny.
    EMBO J. 1984 Aug;3(8):1681-9 PMID: 16453538
  22. Thermo-induced transcripts of a soybean heat shock gene after transfer into sunflower using a Ti plasmid vector.
    EMBO J. 1985 May;4(5):1119-24 PMID: 16453610
  23. Construction of a heat-inducible gene for plants. Demonstration of heat-inducible activity of the Drosophila hsp70 promoter in plants.
    EMBO J. 1985 Nov;4(11):2739-43 PMID: 16453638
  24. The structure and expression of maize genes encoding the major heat shock protein, hsp70.
    EMBO J. 1986 Mar;5(3):451-8 PMID: 16453670
  25. Organ-specific and light-induced expression of plant genes.
    Science. 1986 May 30;232(4754):1106-12 PMID: 17754498
  26. Duplications of a mutated simian virus 40 enhancer restore its activity.
    Nature. 1985 Feb 21-27;313(6004):711-4 PMID: 2983220
  27. Binary Agrobacterium vectors for plant transformation.
    Nucleic Acids Res. 1984 Nov 26;12(22):8711-21 PMID: 6095209
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-05-00
Pages
1161-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553914
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2