Home LiteratureArticle Details
PMID: 16453773 Published · ppublish English Journal Article

Transcription and RNA stability are important determinants of higher plant chloroplast RNA levels.

The EMBO journal ·Vol. 6 ·No. 6 ·1987-06-00 ·Pages 1571-9

Mullet JE, Klein RR

Abstract

Transcription in lysed barley plastids and Northern slot blot analyses were used to determine the relationship between changes in RNA levels and transcription during plastid development. Transcription in plastids of 4.5-9-day-old dark-grown or illuminated barley seedlings declined up to 10-fold as a function of plant age. Decreased transcription of some plastid genes (rbcL, psaA-psaB) was paralleled by decreased levels of mRNA. In other cases (16SrDNA, psbA) the changes in transcription were not followed by proportional changes in RNA levels indicating that RNA stability is important in establishing the amount of plastid RNA for these genes. Further analysis showed that transcription of the plastid rRNA transcription unit is regulated differently than the transcription of protein coding genes such as psbA or rbcL.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mullet J E
Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128, USA.
Klein R R
References (41)
41 references, click to expand
  1. Phytochrome control of levels of mRNA complementary to plastid and nuclear genes of maize.
    Plant Physiol. 1985 Oct;79(2):371-6 PMID: 16664416
  2. Light-stimulated transcription of genes for two chloroplast polypeptides in isolated pea leaf nuclei.
    EMBO J. 1982;1(12):1493-8 PMID: 16453439
  3. Two partially homologous adjacent light-inducible maize chloroplast genes encoding polypeptides of the P700 chlorophyll a-protein complex of photosystem I.
    J Biol Chem. 1985 Feb 10;260(3):1413-21 PMID: 3881431
  4. Light-induced de Novo Synthesis of Ribulose 1,5-Diphosphate Carboxylase in Greening Leaves of Barley.
    Plant Physiol. 1970 Sep;46(3):416-8 PMID: 16657477
  5. Genome Expression during Normal Leaf Development : I. CELLULAR AND CHLOROPLAST NUMBERS AND DNA, RNA, AND PROTEIN LEVELS IN TISSUES OF DIFFERENT AGES WITHIN A SEVEN-DAY-OLD WHEAT LEAF.
    Plant Physiol. 1982 Apr;69(4):904-10 PMID: 16662317
  6. Ribonucleic acid synthesizing activity of spinach chloroplasts and nuclei.
    Arch Biochem Biophys. 1967 Aug;121(2):336-45 PMID: 6057103
  7. Vasodilator response in the lower extremity induced by contrast medium. I Canine model.
    Acta Radiol Diagn (Stockh). 1982;23(2):81-6 PMID: 7090855
  8. Ribonucleic acid synthesis in chloroplasts.
    Biochem J. 1973 May;134(1):249-62 PMID: 4723226
  9. Differential transcription in vivo and in vitro of two adjacent maize chloroplast genes: The large subunit of ribulosebisphosphate carboxylase and the 2.2-kilobase gene.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6821-5 PMID: 16593121
  10. The Nature of Nucleotide Sequence Divergence between Barley and Maize Chloroplast DNA.
    Genetics. 1984 Apr;106(4):735-49 PMID: 17246206
  11. The light-dependent accumulation of the P700 chlorophyll a protein of the photosystem I reaction center in barley. Evidence for translational control.
    Eur J Biochem. 1986 Sep 15;159(3):459-67 PMID: 3530758
  12. Control of gene expression during higher plant chloroplast biogenesis. Protein synthesis and transcript levels of psbA, psaA-psaB, and rbcL in dark-grown and illuminated barley seedlings.
    J Biol Chem. 1987 Mar 25;262(9):4341-8 PMID: 3558409
  13. Transcriptional activity of isolated maize chloroplasts.
    Arch Biochem Biophys. 1984 Nov 15;235(1):26-33 PMID: 6208848
  14. Rapid transfer of DNA from agarose gels to nylon membranes.
    Nucleic Acids Res. 1985 Oct 25;13(20):7207-21 PMID: 4059056
  15. Biosynthesis of chloroplast transfer RNA in a spinach chloroplast transcription system.
    Cell. 1983 Dec;35(3 Pt 2):815-28 PMID: 6652686
  16. Differential expression of the gene for the large subunit of ribulose bisphosphate carboxylase in maize leaf cell types.
    Cell. 1978 Nov;15(3):725-31 PMID: 728987
  17. Preferential transcription of cloned maize chloroplast DNA sequences by maize chloroplast RNA polymerase.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):822-6 PMID: 16592779
  18. Light-induced increase in the number and activity of ribosomes bound to pea chloroplast thylakoids in vivo.
    Plant Physiol. 1982 Apr;69(4):814-24 PMID: 16662302
  19. An in vitro system for accurate transcription initiation of chloroplast protein genes.
    Nucleic Acids Res. 1985 Feb 25;13(4):1283-302 PMID: 2860636
  20. The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.
    EMBO J. 1986 Sep;5(9):2043-2049 PMID: 16453699
  21. Selective in vitro transcription of Euglena chloroplast ribosomal RNA genes by a transcriptionally active chromosome.
    J Biol Chem. 1980 Apr 25;255(8):3786-92 PMID: 6767727
  22. Characterization of a Euglena gracilis chloroplast RNA polymerase specific for ribosomal RNA genes.
    J Biol Chem. 1985 Sep 15;260(20):11194-9 PMID: 3928629
  23. Regulation of ribosome production in Escherichia coli: synthesis and stability of ribosomal RNA and of ribosomal protein messenger RNA at different growth rates.
    J Mol Biol. 1977 Sep 25;115(3):335-54 PMID: 338910
  24. Isolation of a transcriptionally active chromosome from chloroplasts of Euglena gracilis.
    Biochemistry. 1976 Jul 13;15(14):3039-45 PMID: 821516
  25. Adenine nucleotide translocation in spinach chloroplasts.
    FEBS Lett. 1969 Sep;5(1):11-14 PMID: 11947226
  26. Stringent control in E. coli.
    Annu Rev Genet. 1979;13:393-415 PMID: 94252
  27. Structure and function of developing barley plastids.
    Plant Physiol. 1974 Aug;54(2):148-59 PMID: 16658850
  28. Differential regulation of the accumulation of the light-harvesting chlorophyll a/b complex and ribulose bisphosphate carboxylase/oxygenase in greening pea leaves.
    J Cell Biochem. 1984;25(1):1-13 PMID: 6470048
  29. Regulation of chloroplast-encoded chlorophyll-binding protein translation during higher plant chloroplast biogenesis.
    J Biol Chem. 1986 Aug 25;261(24):11138-45 PMID: 3525563
  30. Evidence for two RNA polymerase activities in Euglena gracilis chloroplasts.
    J Biol Chem. 1984 Dec 10;259(23):14880-7 PMID: 6094579
  31. Maize plastid photogenes: mapping and photoregulation of transcript levels during light-induced development.
    J Cell Biol. 1985 Feb;100(2):463-76 PMID: 2981888
  32. DNA supercoiling affects in vitro transcription of two maize chloroplast genes differently.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):4886-90 PMID: 3860830
  33. Characterization of transcriptionally active DNA-protein complexes from chloroplasts and etioplasts of mustard (Sinapis alba L.).
    Eur J Biochem. 1985 Apr 15;148(2):207-12 PMID: 2580705
  34. Rapid induction of selective transcription by auxins.
    Mol Cell Biol. 1985 Jun;5(6):1197-203 PMID: 4041007
  35. Transcription activity of a DNA-protein complex isolated from spinach plastids.
    Eur J Biochem. 1979 Jul;98(1):285-92 PMID: 467444
  36. Sizes, locations, and directions of transcription of two genes on a cloned maize chloroplast DNA sequence.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1832-6 PMID: 16592800
  37. Mutational analysis of the maize chloroplast ATPase-beta subunit gene promoter: the isolation of promoter mutants in E. coli and their characterization in a chloroplast in vitro transcription system.
    EMBO J. 1985 Dec 30;4(13B):3641-8 PMID: 3004965
  38. Analysis of promoter regions for the spinach chloroplast rbcL, atpB and psbA genes.
    EMBO J. 1985 Dec 16;4(13A):3375-83 PMID: 2868888
  39. Properties and characterization of a spinach chloroplast RNA polymerase isolated from a tanscriptionally active DNA-protein complex.
    Eur J Biochem. 1980 Oct;111(2):503-9 PMID: 7460913
  40. Maize plastid gene expressed during photoregulated development.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3060-4 PMID: 16592541
  41. DNA sequence requirements for the accurate transcription of a protein-coding plastid gene in a plastid in vitro system from mustard (Sinapis alba L.).
    EMBO J. 1984 Aug;3(8):1697-704 PMID: 16453540
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-06-00
Pages
1571-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553526
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2