Protein translocation across wheat germ microsomal membranes requires an SRP-like component.
Different wheat germ extracts were tested for the presence of membranes capable of translocating and processing nascent secretory proteins. One lysate was found in which nascent prehuman-placental lactogen (phPL) was translocated and processed to mature human placental lactogen (hPL). Processing was found to occur concomitant with translocation across membranes. Translocation across the wheat germ membrane required a component which is similar to the mammalian signal recognition particle (SRP). It bound to DEAE-Sepharose, had a sedimentation coefficient of 11S and contained a 7S RNA. In addition to hPL, the plant protein zein and the bacterial protein beta-lactamase were translocated across and processed by wheat germ membranes. Transport was found to occur only co-translationally. Our results show that the wheat germ protein translocation system is similar to the mammalian one. Unlike the mammalian SRP, the particle purified from wheat germ did not arrest elongation of nascent secretory proteins.
25 references, click to expand
-
Protein translocation across and integration into membranes.CRC Crit Rev Biochem. 1986;20(1):73-137 PMID: 3007024
-
Signal recognition particle (SRP) does not mediate a translational arrest of nascent secretory proteins in mammalian cell-free systems.EMBO J. 1985 Aug;4(8):2031-3 PMID: 2415357
-
Secretion in yeast: reconstitution of the translocation and glycosylation of alpha-factor and invertase in a homologous cell-free system.Cell. 1986 Feb 28;44(4):619-28 PMID: 3512097
-
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4 PMID: 4525168
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
-
Protein translocation across the endoplasmic reticulum.Cell. 1984 Aug;38(1):5-8 PMID: 6088076
-
Protein transfer across microsomal membranes reassembled from separated membrane components.Nature. 1978 Jun 15;273(5663):569-71 PMID: 96350
-
Post-translational cleavage of presecretory proteins with an extract of rough microsomes from dog pancreas containing signal peptidase activity.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5598-602 PMID: 271987
-
Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.J Cell Biol. 1975 Dec;67(3):852-62 PMID: 811672
-
Synthesis and processing of maize storage proteins in Xenopus laevis oocytes.Proc Natl Acad Sci U S A. 1979 Dec;76(12):6448-52 PMID: 16592743
-
Signal recognition particle: a ribonucleoprotein required for cotranslational translocation of proteins, isolation and properties.Methods Enzymol. 1983;96:682-91 PMID: 6197610
-
A receptor for single segments of secretory proteins in rough endoplasmic reticulum membranes.FEBS Lett. 1981 Jan 12;123(1):79-84 PMID: 6258987
-
Protein translocation across the endoplasmic reticulum. I. Detection in the microsomal membrane of a receptor for the signal recognition particle.J Cell Biol. 1982 Nov;95(2 Pt 1):463-9 PMID: 6292235
-
Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.Proc Natl Acad Sci U S A. 1980 Dec;77(12):7112-6 PMID: 6938958
-
Secretory protein translocation across membranes-the role of the "docking protein'.Nature. 1982 Jun 24;297(5868):647-50 PMID: 7088152
-
Identification and characterization of a membrane component essential for the translocation of nascent proteins across the membrane of the endoplasmic reticulum.J Cell Biol. 1980 NOV;87(2 Pt 1):503-8 PMID: 7430254
-
Translation efficiency of zein mRNA is reduced by hybrid formation between the 5'- and 3'-untranslated region.EMBO J. 1985 Sep;4(9):2153-8 PMID: 15938048
-
Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.J Cell Biol. 1986 May;102(5):1543-50 PMID: 3517001
-
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
-
Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum.Nature. 1982 Oct 21;299(5885):691-8 PMID: 6181418
-
Signal recognition particle triggers the translocation of storage globulin polypeptides from field beans (Vicia faba L.) across mammalian endoplasmic reticulum membrane.FEBS Lett. 1984 Jan 30;166(2):314-20 PMID: 6198216
-
Isolation of the Escherichia coli leader peptidase gene and effects of leader peptidase overproduction in vivo.Proc Natl Acad Sci U S A. 1981 Oct;78(10):6106-10 PMID: 6273848
-
Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.J Biol Chem. 1980 Mar 10;255(5):2123-30 PMID: 6986388
-
Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.J Cell Biol. 1981 Nov;91(2 Pt 1):557-61 PMID: 7309797
-
In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent posttranslational translocation of the prepro-alpha-factor.Cell. 1986 May 9;45(3):397-406 PMID: 3009026
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