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PMID: 16455921 Published · ppublish English Evaluation Study Journal Article Research Support, N.I.H., Extramural

Proximal region of the gene encoding cytadherence-related protein permits molecular typing of Mycoplasma genitalium clinical strains by PCR-restriction fragment length polymorphism.

Journal of clinical microbiology ·Vol. 44 ·No. 2 ·2006-02-00 ·Pages 598-603

Musatovova O, Herrera C, Baseman JB

Abstract

Restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified proximal region of the gene encoding cytadherence accessory protein P110 (MG192) revealed DNA sequence divergences among 54 Mycoplasma genitalium clinical strains isolated from the genitourinary tracts of women attending a sexually transmitted disease-related health clinic, plus one from the respiratory tract and one from synovial fluid. Seven of 56 (12.5%) strains exhibited RFLPs following digestion of the proximal region with restriction endonuclease MboI or RsaI, or both. No sequence variability was detected in the distal portion of the gene.

MeSH Terms
Bacterial Adhesion Bacterial Proteins/chemistry,genetics Bacterial Typing Techniques Female Humans Membrane Proteins/chemistry,genetics Molecular Sequence Data Mycoplasma Infections/microbiology Mycoplasma genitalium/classification,genetics Polymerase Chain Reaction/methods Polymorphism, Restriction Fragment Length Sequence Analysis, DNA
Chemicals
Bacterial Proteins HMW1 protein, Mycoplasma Membrane Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Musatovova Oxana
Department of Microbiology and Immunology, MC 7758, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA.
Herrera Caleb
Baseman Joel B
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.44.2.598-603.2006
Published
2006-02-00
Pages
598-603
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC1392663
Subset
IM
Grants
NIAID NIH HHS · R01 AI041010 · United States
NIAID NIH HHS · U19 AI045429 · United States
NIAID NIH HHS · AI041010-06 · United States
NIAID NIH HHS · AI45429-06 · United States
Databases
GENBANK
AY679761
External Links
PubMed source DOI: 10.1128/JCM.44.2.598-603.2006 PMC Full Text View journal details
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