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PMID: 164667 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Decrease in membrane-associated actin of fibroblasts after transformation by Rous sarcoma virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 2 ·1975-02-00 ·Pages 746-9

Wickus G, Gruenstein E, Robbins PW, Rich A

Abstract

The actin content of membranes prepared from cultured chick embryo fibroblasts has been measured on polyacrylamide gels. The actin was identified by tryptic peptide mapping. After transformation of the cells by Rous sarcoma virus, the amount of actin associated with the membranes is decreased by 30-50%. This result is not due to infection per se, since infection by a temperature-sensitive strain of the virus decreases membrane-associated actin only at the permissive temperature. A shift from the nonpermissive (41 degrees) to the permissive (36 degrees) temperature results in an increase in the percentage of total cellular protein synthesis devoted to actin production, so that the decrease in membrane-associated actin appears to be a selective displacement from the membrane rather than a general decrease in total cellular actin.

MeSH Terms

Actins/analysis,biosynthesis Animals Avian Sarcoma Viruses Cell Membrane/analysis Cell Transformation, Neoplastic Cells, Cultured Chick Embryo Fibroblasts Methionine/metabolism Temperature

Chemicals

Actins Methionine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wickus G

Gruenstein E

Robbins P W

Rich A

References (25)

25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.2.746

Published

1975-02-00

Pages

746-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC432393

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.2.746 PMC Full Text View journal details

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Decrease in membrane-associated actin of fibroblasts after transformation by Rous sarcoma virus.

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