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PMID: 16469845 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Homotypic fusion of early endosomes: SNAREs do not determine fusion specificity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 8 ·2006-02-21 ·Pages 2701-6

Brandhorst D, Zwilling D, Rizzoli SO, Lippert U, Lang T, Jahn R

Abstract

Membrane fusion in the secretory pathway is mediated by soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins. Different fusion steps are thought to be effected by independent sets of SNAREs, but it is unclear whether specificity is determined by an intrinsic specificity of SNARE pairing or by upstream factors. Using a newly developed microscopy-based assay, we have investigated the SNARE specificity of homotypic early endosomal fusion. We show that early endosomes contain multiple sets of SNAREs, including, in addition to the putative early endosomal SNAREs, those involved in exocytosis and in fusion of late endosomes. We demonstrate that fusion is largely mediated by a complex formed by syntaxin 13, syntaxin 6, vti1a, and VAMP4, whereas the exocytic and late endosomal SNAREs play little or no role in the reaction. In contrast, proteoliposomes reconstituted with early endosomal SNAREs promiscuously fuse with liposomes containing exocytotic or late endosomal SNAREs. We conclude that the specificity of SNARE pairing does not suffice to determine the specificity of organelle fusion.

MeSH Terms
Animals Endosomes/chemistry,physiology,ultrastructure Membrane Fusion/drug effects PC12 Cells Rats SNARE Proteins/analysis,genetics,metabolism
Chemicals
SNARE Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Brandhorst Dorothea
Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany.
Zwilling Daniel
Rizzoli Silvio O
Lippert Undine
Lang Thorsten
Jahn Reinhard
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0511138103
Published
2006-02-21
Epub
2006-00-09
Pages
2701-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1413832
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0511138103 PMC Full Text View journal details
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