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PMID: 16495572 Published · ppublish English Journal Article

Routine markerless gene replacement in Bacillus anthracis.

Infection and immunity ·Vol. 74 ·No. 3 ·2006-03-00 ·Pages 1949-53

Janes BK, Stibitz S

Abstract

An improved genetic tool suitable for routine markerless allelic exchange in Bacillus anthracis has been constructed. Its utility was demonstrated by the introduction of insertions, deletions, and missense mutations on the chromosome and plasmid pXO1 of the Sterne strain of B. anthracis.

MeSH Terms
Bacillus anthracis/genetics Genes, Bacterial/genetics Genetic Markers Genome, Bacterial Plasmids/genetics
Chemicals
Genetic Markers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Janes Brian K
Division of Bacterial Products, CBER/FDA, 8800 Rockville Pike, Bethesda, MD 20892, USA.
Stibitz Scott
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.74.3.1949-1953.2006
Published
2006-03-00
Pages
1949-53
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC1418658
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.74.3.1949-1953.2006 PMC Full Text View journal details
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