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PMID: 1650452 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

GATA-1 transactivates erythropoietin receptor gene, and erythropoietin receptor-mediated signals enhance GATA-1 gene expression.

Nucleic acids research ·Vol. 19 ·No. 14 ·1991-07-25 ·Pages 3843-8

Chiba T, Ikawa Y, Todokoro K

Abstract

Erythropoietin is a cytokine which specifically regulates the proliferation and differentiation of erythroid progenitor cells. The expression of erythropoietin receptor on the cell membrane of the progenitor cells is a critical event during the erythroid differentiation process. In order to clarify the tissue-specific and differentiation stage-specific expression of the erythropoietin receptor gene, its transcriptional regulation was examined by transient expression assay, gel mobility shift assay and DNase I footprinting. The results clearly showed that GATA-1 transactivates the gene expression through a single GATA motif located around -200 bp upstream from the ATG codon in a dose dependent manner. Furthermore, Northern blot analysis revealed that erythropoietin receptor-mediated signals strongly enhanced GATA-1 gene expression in accordance with the appearance of hemoglobin-positive cells. Taken together with other observations, these results suggested the following scheme of erythroid differentiation: 1)GATA-1 is expressed in the early stage of blood cell development; 2) GATA-1 transactivates the erythropoietin receptor gene; 3) erythropoietin binds its receptor and the receptor-mediated signals enhance GATA-1 gene expression in erythroid progenitor cells; and 4) GATA-1 finally transactivates hemoglobin synthesis-related genes and globin genes in relatively matured erythroid cells.

Related Genes

EpoR GATA-1

MeSH Terms

Animals Base Sequence Cloning, Molecular DNA DNA-Binding Proteins/genetics,metabolism Deoxyribonuclease I/metabolism Erythroid-Specific DNA-Binding Factors Erythropoietin/metabolism GATA1 Transcription Factor Gene Expression Regulation Hematopoiesis/genetics Leukemia, Erythroblastic, Acute Mice Molecular Sequence Data Promoter Regions, Genetic Receptors, Cell Surface/genetics,metabolism Receptors, Erythropoietin Restriction Mapping Signal Transduction Transcription Factors/genetics,metabolism Transcriptional Activation Tumor Cells, Cultured

Chemicals

DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor Gata1 protein, mouse Receptors, Cell Surface Receptors, Erythropoietin Transcription Factors Erythropoietin DNA Deoxyribonuclease I

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Chiba T

Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.

Ikawa Y

Todokoro K

References (29)

29 references, click to expand

Globin gene regulation and switching: circa 1990.
Cell. 1990 Nov 16;63(4):665-72 PMID: 2225071
Activity and tissue-specific expression of the transcription factor NF-E1 multigene family.
Genes Dev. 1990 Oct;4(10):1650-62 PMID: 2249770
Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1.
Nature. 1991 Jan 17;349(6306):257-60 PMID: 1987478
Characterization of murine erythropoietin receptor genes.
J Mol Biol. 1990 Dec 5;216(3):567-75 PMID: 2175360
Transmembrane signaling during erythropoietin- and dimethylsulfoxide-induced erythroid cell differentiation.
Eur J Biochem. 1990 Oct 5;193(1):163-8 PMID: 2171931
The human beta-globin promoter; nuclear protein factors and erythroid specific induction of transcription.
EMBO J. 1988 Dec 20;7(13):4203-12 PMID: 3243278
Increased gamma-globin expression in a nondeletion HPFH mediated by an erythroid-specific DNA-binding factor.
Nature. 1989 Mar 30;338(6214):435-8 PMID: 2467208
Cis- and trans-acting elements involved in the regulation of the erythroid promoter of the human porphobilinogen deaminase gene.
Proc Natl Acad Sci U S A. 1989 Sep;86(17):6548-52 PMID: 2771941
SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat.
Mol Cell Biol. 1988 Jan;8(1):466-72 PMID: 2827008
Expression of high affinity receptors for erythropoietin on human bone marrow cells and on the human erythroleukemic cell line, HEL.
Exp Hematol. 1988 Nov;16(10):836-42 PMID: 2844573
Specific binding of erythropoietin to its receptor on responsive mouse erythroleukemia cells.
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4126-30 PMID: 3035570
The human beta-globin gene 3' enhancer contains multiple binding sites for an erythroid-specific protein.
Genes Dev. 1988 Sep;2(9):1089-100 PMID: 2461328
Expression cloning of the murine erythropoietin receptor.
Cell. 1989 Apr 21;57(2):277-85 PMID: 2539263
Multiple GF-1 binding sites flank the erythroid specific transcription unit of the human carbonic anhydrase I gene.
FEBS Lett. 1989 Nov 6;257(2):451-6 PMID: 2511043
The erythroid-specific transcription factor Eryf1: a new finger protein.
Cell. 1989 Sep 8;58(5):877-85 PMID: 2776214
Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
Down-regulation of c-myb gene expression is a prerequisite for erythropoietin-induced erythroid differentiation.
Proc Natl Acad Sci U S A. 1988 Dec;85(23):8900-4 PMID: 3194397
Expression of an erythroid transcription factor in megakaryocytic and mast cell lineages.
Nature. 1990 Mar 29;344(6265):444-7 PMID: 2320112
Megakaryocytic and erythrocytic lineages share specific transcription factors.
Nature. 1990 Mar 29;344(6265):447-9 PMID: 2320113
Synergy between the NF-E1 erythroid-specific transcription factor and the CACCC factor in the erythroid-specific promoter of the human porphobilinogen deaminase gene.
Mol Cell Biol. 1990 Jul;10(7):3838-42 PMID: 2355926
Structure and transcription of the mouse erythropoietin receptor gene.
Mol Cell Biol. 1990 Jul;10(7):3675-82 PMID: 2162479
Structure and evolution of a human erythroid transcription factor.
Nature. 1990 Jan 4;343(6253):92-6 PMID: 2104960
A nonerythroid GATA-binding protein is required for function of the human preproendothelin-1 promoter in endothelial cells.
Mol Cell Biol. 1990 Sep;10(9):4854-62 PMID: 2388628
The major human erythroid DNA-binding protein (GF-1): primary sequence and localization of the gene to the X chromosome.
Proc Natl Acad Sci U S A. 1990 Jan;87(2):668-72 PMID: 2300555
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54 PMID: 942051

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/19.14.3843

Published

1991-07-25

Pages

3843-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC328472

Subset

External Links

PubMed source DOI: 10.1093/nar/19.14.3843 PMC Full Text View journal details

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GATA-1 transactivates erythropoietin receptor gene, and erythropoietin receptor-mediated signals enhance GATA-1 gene expression.

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