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PMID: 16507586 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Drosophila Incenp is required for cytokinesis and asymmetric cell division during development of the nervous system.

Journal of cell science ·Vol. 119 ·No. Pt 6 ·2006-03-15 ·Pages 1144-53

Chang CJ, Goulding S, Adams RR, Earnshaw WC, Carmena M

Abstract

The chromosomal passenger protein complex has emerged as a key player in mitosis, with important roles in chromatin modifications, kinetochore-microtubule interactions, chromosome bi-orientation and stability of the bipolar spindle, mitotic checkpoint function, assembly of the central spindle and cytokinesis. The inner centromere protein (Incenp; a subunit of this complex) is thought to regulate the Aurora B kinase and target it to its substrates. To explore the roles of the passenger complex in a developing multicellular organism, we have performed a genetic screen looking for new alleles and interactors of Drosophila Incenp. We have isolated a new null allele of Incenp that has allowed us for the first time to study the functions of the chromosomal passengers during development. Homozygous incenp(EC3747) embryos show absence of phosphorylation of histone H3 in mitosis, failure of cytokinesis and polyploidy, and defects in peripheral nervous system development. These defects are consistent with depletion of Aurora B kinase activity. In addition, the segregation of the cell-fate determinant Prospero in asymmetric neuroblast division is abnormal, suggesting a role for the chromosomal passenger complex in the regulation of this process.

MeSH Terms
Animals Aurora Kinases Cell Division/genetics Chromosomal Proteins, Non-Histone/genetics Cytokinesis/genetics Drosophila/embryology Drosophila Proteins/genetics Gene Expression Regulation, Developmental Nervous System/embryology,metabolism Organogenesis Protein Serine-Threonine Kinases/genetics
Chemicals
Chromosomal Proteins, Non-Histone Drosophila Proteins Incenp protein, Drosophila Aurora Kinases Protein Serine-Threonine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chang Chih-Jui
Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Kings Buildings, Mayfield Road, Edinburgh, EH9 3JR, UK.
Goulding Sarah
Adams Richard R
Earnshaw William C
Carmena Mar
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Article Info
Journal
Journal of cell science
Abbr.
J Cell Sci
ISSN
0021-9533
DOI
10.1242/jcs.02834
Published
2006-03-15
Epub
2006-00-28
Pages
1144-53
Language
English
Region
England
NLM ID
0052457
PMCID
PMC7115963
Subset
IM
Grants
Wellcome Trust · United Kingdom
Wellcome Trust · 043189 · United Kingdom
Wellcome Trust · 073915 · United Kingdom
External Links
PubMed source DOI: 10.1242/jcs.02834 PMC Full Text View journal details
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