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PMID: 16514417 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription.

EMBO reports ·Vol. 7 ·No. 5 ·2006-05-00 ·Pages 525-30

Percipalle P, Fomproix N, Cavellán E, Voit R, Reimer G, Krüger T, Thyberg J, Scheer U, Grummt I, Farrants AK

Abstract

Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein-protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.

MeSH Terms
Adenosine Triphosphatases/chemistry,genetics,metabolism Cell Nucleus/chemistry,genetics,metabolism Chromatin Assembly and Disassembly/genetics Chromosomal Proteins, Non-Histone/chemistry,genetics,metabolism HeLa Cells Humans Intracellular Signaling Peptides and Proteins/genetics,metabolism,physiology Myosin Type I/chemistry,genetics,metabolism Nuclear Proteins/genetics,metabolism,physiology Protein Binding/genetics RNA Polymerase I/biosynthesis,chemistry,genetics Transcription Factors/chemistry,genetics,metabolism Transcription, Genetic
Chemicals
BAZ1B protein, human Chromosomal Proteins, Non-Histone Intracellular Signaling Peptides and Proteins NMI protein, human Nuclear Proteins Transcription Factors RNA Polymerase I Adenosine Triphosphatases Myosin Type I SMARCA5 protein, human MYO1C protein, human
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Percipalle Piergiorgio
Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, Box 285, Stockholm 171 77, Sweden. [email protected]
Fomproix Nathalie
Cavellán Erica
Voit Renate
Reimer Georg
Krüger Tim
Thyberg Johan
Scheer Ulrich
Grummt Ingrid
Farrants Ann-Kristin Ostlund
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29 references, click to expand
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1038/sj.embor.7400657
Email
[email protected]
Published
2006-05-00
Epub
2006-00-03
Pages
525-30
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1479564
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.embor.7400657 PMC Full Text View journal details
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