Home LiteratureArticle Details
PMID: 1651478 Published · ppublish English Journal Article

A new cAMP response element in the transcribed region of the human c-fos gene.

Nucleic acids research ·Vol. 19 ·No. 15 ·1991-08-11 ·Pages 4153-9

Härtig E, Loncarević IF, Büscher M, Herrlich P, Rahmsdorf HJ

Abstract

In NIH 3T3 cells the c-fos gene is induced rapidly and transiently by cAMP. As shown by the analysis of 3T3 cells stably transfected with promoter mutants of the human c-fos gene this induction does not depend on the dyad symmetry element (position -320 to -300), but involves at least two other non-related sites: an element located around position -60 resembling the cAMP response element of the fibronectin and somatostatin genes (which has been described before), and an element located between positions +18 and +38. Destruction of one or the other element in the c-fos gene reduces cAMP inducibility. The cAMP response of c-fos promoter CAT gene constructs also depends on these elements in transient transfection assays. When cloned in front of the albumin TATA box, both elements independently mediate cAMP inducibility. These elements do not bind the same protein as shown in gel retardation analyses, suggesting that two different cAMP inducible factors mediate the activation of the c-fos gene by cAMP.

Related Genes
c-fos
MeSH Terms
Animals Base Sequence Cell Line Cyclic AMP/metabolism Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins/genetics Gene Expression Regulation/physiology Humans Molecular Sequence Data Promoter Regions, Genetic/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-fos Proto-Oncogenes/genetics Recombinant Fusion Proteins/biosynthesis Regulatory Sequences, Nucleic Acid Transcription, Genetic Transfection/genetics
Chemicals
Cyclic AMP Response Element-Binding Protein DNA-Binding Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Recombinant Fusion Proteins Cyclic AMP
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Härtig E
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, FRG.
Loncarević I F
Büscher M
Herrlich P
Rahmsdorf H J
References (51)
51 references, click to expand
  1. Induction of c-fos gene and protein by growth factors precedes activation of c-myc.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):716-20 PMID: 6334806
  2. 'Nuclear' oncogenes convert extracellular stimuli into changes in the genetic program.
    Trends Genet. 1989 Apr;5(4):112-5 PMID: 2658225
  3. The heat shock response in HeLa cells is accompanied by elevated expression of the c-fos proto-oncogene.
    Mol Cell Biol. 1987 Oct;7(10):3452-8 PMID: 3316977
  4. Transcriptional arrest within the first exon is a fast control mechanism in c-myc gene expression.
    Nucleic Acids Res. 1986 Nov 11;14(21):8331-46 PMID: 3537956
  5. Metal-responsive elements act as positive modulators of human metallothionein-IIA enhancer activity.
    Mol Cell Biol. 1987 Feb;7(2):606-13 PMID: 3821726
  6. Induction of the c-fos oncogene by thyrotropic hormone in rat thyroid cells in culture.
    Science. 1986 Jul 25;233(4762):458-60 PMID: 3726540
  7. Antitumor promotion and antiinflammation: down-modulation of AP-1 (Fos/Jun) activity by glucocorticoid hormone.
    Cell. 1990 Sep 21;62(6):1189-204 PMID: 2169351
  8. Transcription factors: a new family gathers at the cAMP response site.
    Trends Genet. 1990 Mar;6(3):69-72 PMID: 2158162
  9. Demonstration in living cells of an intragenic negative regulatory element within the rodent c-fos gene.
    Cell. 1990 May 4;61(3):485-96 PMID: 2110508
  10. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  11. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  12. Immunoglobulin gene transcription is activated by downstream sequence elements.
    Cell. 1983 Jul;33(3):741-8 PMID: 6409419
  13. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
    Cell. 1983 Jul;33(3):729-40 PMID: 6409418
  14. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
    Cell. 1983 Jul;33(3):717-28 PMID: 6409417
  15. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  16. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  17. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  18. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
  19. The first intron of the alpha 1(I) collagen gene contains several transcriptional regulatory elements.
    J Biol Chem. 1988 Feb 5;263(4):1603-6 PMID: 2828347
  20. Sequence requirements for premature termination of transcription in the human c-myc gene.
    Cell. 1988 Apr 22;53(2):245-56 PMID: 2834065
  21. Properties of the human hepatitis B virus enhancer: position effects and cell-type nonspecificity.
    J Virol. 1988 Apr;62(4):1305-13 PMID: 2831391
  22. Multiple basal promoter elements determine the level of human c-fos transcription.
    Mol Cell Biol. 1991 Mar;11(3):1270-80 PMID: 1899906
  23. Regulation of HIV gene expression by RNA-protein interactions.
    Trends Genet. 1991 Jan;7(1):9-14 PMID: 2003337
  24. Hepatocyte-specific promoter element HP1 of the Xenopus albumin gene interacts with transcriptional factors of mammalian hepatocytes.
    J Mol Biol. 1988 Jul 20;202(2):307-20 PMID: 3172219
  25. Identification of c-fos sequences involved in induction by insulin and phorbol esters.
    J Biol Chem. 1988 Feb 5;263(4):1611-4 PMID: 3276673
  26. c-fos sequence necessary for basal expression and induction by epidermal growth factor, 12-O-tetradecanoyl phorbol-13-acetate and the calcium ionophore.
    Mol Cell Biol. 1987 Oct;7(10):3490-502 PMID: 3119989
  27. Induction of c-fos and c-myc mRNA by epidermal growth factor or calcium ionophore is cAMP dependent.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8216-20 PMID: 2430281
  28. Autoregulation of fos: the dyad symmetry element as the major target of repression.
    EMBO J. 1989 Sep;8(9):2559-66 PMID: 2511006
  29. Activation of the c-fos gene by UV and phorbol ester: different signal transduction pathways converge to the same enhancer element.
    Oncogene. 1988 Sep;3(3):301-11 PMID: 2849742
  30. Induction of proto-oncogene fos transcription through the adenylate cyclase pathway: characterization of a cAMP-responsive element.
    Genes Dev. 1988 Dec;2(12A):1529-38 PMID: 2850967
  31. The Fos and Jun/AP-1 proteins are involved in the downregulation of Fos transcription.
    Oncogene. 1989 May;4(5):629-36 PMID: 2498806
  32. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements.
    Nucleic Acids Res. 1987 Jul 10;15(13):5490 PMID: 3037497
  33. Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP.
    Cell. 1987 Oct 23;51(2):251-60 PMID: 2822255
  34. Human immunodeficiency virus type 1 LTR TATA and TAR region sequences required for transcriptional regulation.
    EMBO J. 1989 Mar;8(3):765-78 PMID: 2721501
  35. Structural arrangements of transcription control domains within the 5'-untranslated leader regions of the HIV-1 and HIV-2 promoters.
    Genes Dev. 1988 Sep;2(9):1101-14 PMID: 2847959
  36. The HeLa cell protein TEF-1 binds specifically and cooperatively to two SV40 enhancer motifs of unrelated sequence.
    Cell. 1988 Sep 23;54(7):931-42 PMID: 2843293
  37. Involvement of common and cell type-specific pathways in c-fos gene control: stable induction of cAMP in macrophages.
    Cell. 1987 Jan 30;48(2):251-60 PMID: 3026638
  38. Intragenic pausing and anti-sense transcription within the murine c-myc locus.
    EMBO J. 1986 Nov;5(11):2859-65 PMID: 3024965
  39. 12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5'-flanking region.
    Mol Cell Biol. 1987 Jun;7(6):2256-66 PMID: 3037355
  40. Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.
    Science. 1988 Dec 9;242(4884):1430-3 PMID: 2974179
  41. Calcium and growth factor pathways of c-fos transcriptional activation require distinct upstream regulatory sequences.
    Mol Cell Biol. 1988 Jul;8(7):2787-96 PMID: 3136322
  42. Posttranscriptional regulation of c-fos mRNA expression.
    Nucleic Acids Res. 1987 Feb 25;15(4):1643-59 PMID: 3103102
  43. The c-fos serum response element responds to protein kinase C-dependent and -independent signals but not to cyclic AMP.
    Genes Dev. 1988 Apr;2(4):394-402 PMID: 2453393
  44. Role of ion flux in the control of c-fos expression.
    Nature. 1986 Aug 7-13;322(6079):552-5 PMID: 2426600
  45. Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences.
    Cell. 1985 Oct;42(3):889-902 PMID: 2414012
  46. UV-induced DNA damage is an intermediate step in UV-induced expression of human immunodeficiency virus type 1, collagenase, c-fos, and metallothionein.
    Mol Cell Biol. 1989 Nov;9(11):5169-81 PMID: 2557547
  47. Multiple sequence elements of a single functional class are required for cyclic AMP responsiveness of the mouse c-fos promoter.
    Mol Cell Biol. 1989 Oct;9(10):4272-81 PMID: 2555687
  48. Forskolin inducibility and tissue-specific expression of the fibronectin promoter.
    Mol Cell Biol. 1989 Apr;9(4):1498-506 PMID: 2542772
  49. Multiple sequence elements in the c-fos promoter mediate induction by cAMP.
    Genes Dev. 1989 Feb;3(2):198-211 PMID: 2541049
  50. A cluster of phosphorylation sites on the cyclic AMP-regulated nuclear factor CREB predicted by its sequence.
    Nature. 1989 Feb 23;337(6209):749-52 PMID: 2521922
  51. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
    Nature. 1984 Oct 4-10;311(5985):433-8 PMID: 6090941
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.15.4153
Published
1991-08-11
Pages
4153-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328555
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.15.4153 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2