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PMID: 16525062 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

RE-1 silencer of transcription/neural restrictive silencer factor modulates ectodermal patterning during Xenopus development.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 26 ·No. 10 ·2006-03-08 ·Pages 2820-9

Olguín P, Oteíza P, Gamboa E, Gómez-Skármeta JL, Kukuljan M

Abstract

RE-1 silencer of transcription/neural restrictive silencer factor (REST/NRSF), a transcriptional repressor, binds to the RE-1 element present in many vertebrate genes. In vitro studies indicate that REST/NRSF plays important roles in several stages of neural development. However, a full understanding of its physiological function requires in vivo approaches. We find that impairment of REST/NRSF function in Xenopus embryos leads to the perturbation of neural tube, cranial ganglia, and eye development. The origin of these defects is the abnormal patterning of the ectoderm during gastrulation. Interference of REST/NRSF function during the late blastula stage leads to an expansion of the neural plate, concomitant with a decrease of the expression of epidermal keratin and neural crest markers. Furthermore, neurogenesis proceeds abnormally, with loss of the expression of proneural, neurogenic, and neuronal genes. The interference of REST/NRSF mimics several features associated with a decreased bone morphogenetic protein (BMP) function and counteracts some effects of BMP4 misexpression. Our results indicate that REST/NRSF function is required in vivo for the acquisition of specific ectodermal cell fates.

MeSH Terms
Animals Body Patterning/drug effects,physiology Ectoderm/metabolism Embryo, Nonmammalian Gene Expression Regulation, Developmental/drug effects,physiology Immunohistochemistry/methods In Situ Hybridization/methods Microinjections/methods Molecular Biology/methods Morphogenesis Neurons/physiology RNA, Messenger/metabolism Repressor Proteins/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction/methods Transcription Factors/genetics,metabolism Transcription, Genetic/drug effects,physiology Xenopus
Chemicals
RE1-silencing transcription factor RNA, Messenger Repressor Proteins Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Olguín Patricio
Centro de Neurociencias Integradas, Iniciativa Científica Milenio, Programa de Fisiología y Biofísica, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, 838-0453 Independencia, Chile. [email protected]
Oteíza Pablo
Gamboa Eduardo
Gómez-Skármeta José Luis
Kukuljan Manuel
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.5037-05.2006
Email
[email protected]
Published
2006-03-08
Pages
2820-9
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6675167
Subset
IM
External Links
PubMed source DOI: 10.1523/JNEUROSCI.5037-05.2006 PMC Full Text View journal details
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