A thermophilic, long-chain fatty acid-oxidizing culture was enriched. Stearate was used as the substrate, and methane and carbon dioxide were the sole end products. Cultivation was possible only when a fed-batch system was used or with addition of activated carbon or bentonite. The enrichment culture consisted of a short rod and two bacteria antigenically related to Methanobacterium thermoautotrophicum (Delta)H and Methanosarcina thermophila TM-1.
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