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PMID: 165531 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

An improved method of fixation for immunofluorescent detection of SV40 T-antigen in infected human fibroblasts.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) ·Vol. 148 ·No. 3 ·1975-03-00 ·Pages 660-4

Kaplan MM, Giard DJ, Blattner WA, Lubiniecki AS, Fraumeni JF

Abstract

Improved fixation for immunofluorescence staining in the SV40 T-antigen test has been developed utilizing ethanol in place of acetone. The modified method improves resolution, results in smaller variability of cell numbers, and reduces the time of assay, while maintaining its sensitivity in discriminating between normal and cancer prone groups.

MeSH Terms

Acetone Animals Antigens, Neoplasm Antigens, Viral Biopsy Cell Line Cell Nucleus/immunology Cells, Cultured Cricetinae/immunology Ethanol Fibroblasts/immunology,microbiology Fluorescent Antibody Technique Formaldehyde Kidney Methanol Methods Rabbits/immunology Simian virus 40/immunology Skin/pathology Temperature

Chemicals

Antigens, Neoplasm Antigens, Viral Acetone Formaldehyde Ethanol Methanol

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Kaplan M M

Giard D J

Blattner W A

Lubiniecki A S

Fraumeni J F

Article Info

Journal

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)

Abbr.

Proc Soc Exp Biol Med

ISSN

0037-9727

DOI

10.3181/00379727-148-38605

Published

1975-03-00

Pages

660-4

Language

English

Region

United States

NLM ID

7505892

Subset

External Links

PubMed source DOI: 10.3181/00379727-148-38605 View journal details

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An improved method of fixation for immunofluorescent detection of SV40 T-antigen in infected human fibroblasts.

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