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PMID: 16557749 Published · ppublish English Journal Article

Role of Glycolipids and Phosphatidylglycerol in the Serological Activity of Mycoplasma pneumoniae.

Infection and immunity ·Vol. 1 ·No. 4 ·1970-04-00 ·Pages 408-16

Razin S, Prescott B, Caldes G, James WD, Chanock RM

Abstract

Mycoplasma pneumoniae lipids were fractionated by combined silicic acid and diethylaminoethyl-cellulose chromatography. The lipid fractions were tested for complement-fixing activity and for blocking of metabolism-inhibiting antibodies in human and rabbit antisera to M. pneumoniae. Thin-layer chromatography and carbohydrate analysis of hydrolysates of the lipid fractions indicated that glycolipids were responsible for the specific serological activities of the lipid extracts of the organism. Phosphatidylglycerol, which accompanied the glycolipids, was serologically inactive when isolated free from glycolipids. Phosphatidylglycerol considerably enhanced the serological activity of the glycolipids, serving as an auxiliary lipid. Because of this auxiliary effect, minute quantities of contaminating glycolipids sufficed to render serological activity to "purified" phosphatidylgylcerol preparations, providing an explanation to the previous identification of the active lipid as a phospholipid.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Razin S
Laboratory of Infectious Diseases and Laboratory of Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014.
Prescott B
Caldes G
James W D
Chanock R M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.1.4.408-416.1970
Published
1970-04-00
Pages
408-16
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415913
External Links
PubMed source DOI: 10.1128/iai.1.4.408-416.1970 PMC Full Text View journal details
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