In vitro assessment of cellular immunity to vaccinia virus: contribution of lymphocytes and macrophages.

Infection and immunity ·Vol. 2 ·No. 6 ·1970-12-00 ·Pages 783-90

Tompkins WA, Zarling JM, Rawls WE

Abstract

The possible contributions of lymphocytes and macrophages to immunity to vaccinia virus were examined in vitro by determination of antigen-specific inhibition of migration of macrophages and the replication of the virus in purified macrophages. Lymphocytes from various anatomic sites of vaccinia virus-immune rabbits mixed with macrophages from nonimmune rabbits resulted in antigen-specific inhibition of macrophage migration; purified lymphocytes of peritoneal exudates were found to be the most potent inhibitors. Macrophages from peritoneal exudates of immune rabbits purified to contain less than 1% lymphocytes were sensitive to inhibition of migration by vaccinia antigens. Virus grew in macrophages from nonimmune rabbits, but failed to replicate in macrophages from the peritoneal cavity of immune rabbits. Alveolar maracrophages from immune rabbits were not inhibited from migrating in the presence of the viral antigen, and the virus replicated in these cells. The data suggest, but do not prove, that cellular immunity to vaccinia virus in rabbits may be mediated both by lymphocytes and by macrophages.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Tompkins W A

Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77025.

Zarling J M

Rawls W E

References (26)

26 references, click to expand

Study of delayed hypersensitivity to myxoviruses induced by vaccines.
Appl Microbiol. 1969 Apr;17(4):577-83 PMID: 4306260
Interferon production by mouse peritoneal cells.
J Reticuloendothel Soc. 1970 Jan;7(1):32-42 PMID: 4314245
A human interferon that crosses the species line.
Proc Natl Acad Sci U S A. 1968 Jan;59(1):69-76 PMID: 4298335
Replication of poliovirus in phytohemagglutinin-stimulated human lymphocytes.
J Virol. 1969 May;3(5):451-7 PMID: 4306718
Protection against poxviruses.
Proc R Soc Med. 1969 Mar 3;62(3):295-7 PMID: 4307230
Macrophages and viral immunity.
Semin Hematol. 1970 Apr;7(2):185-214 PMID: 4317324
Relationships between viruses and leucocytes.
Prog Med Virol. 1966;8:62-130 PMID: 4959446
An in vitro measure of cellular immunity to fibroma virus.
J Immunol. 1970 Feb;104(2):502-10 PMID: 4904883
The role of the macrophage in delayed hypersensitivity.
Br Med Bull. 1967 Jan;23(1):9-14 PMID: 5342388
Cell-mediated immune responses to virus infections and virus-induced tumours.
Br Med Bull. 1967 Jan;23(1):60-5 PMID: 5342382
Specific suppression of tumor growth by isolated peritoneal macrophages from immunized mice.
J Immunol. 1965 Oct;95(4):656-64 PMID: 5320526
ASPECTS OF THE PATHOGENESIS OF VIRUS DISEASES.
Bacteriol Rev. 1964 Mar;28:30-71 PMID: 14127970
DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS.
J Immunol. 1964 Aug;93:264-73 PMID: 14205621
EFFECT OF IMMUNIZATION ON TISSUE SUSCEPTIBILITY TO VACCINIA VIRUS IN VITRO.
Virology. 1963 Nov;21:402-10 PMID: 14081365
Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction.
Proc Natl Acad Sci U S A. 1966 Jul;56(1):72-7 PMID: 5229858
Contact sensitivity in the mouse. IV. The role of lymphocytes and macrophages in passive transfer and the mechanism of their interaction.
J Exp Med. 1970 Jul 1;132(1):1-15 PMID: 5508245
Cellular deoxyribonucleic acid synthesis and loss of contact inhibition in irradiated and contact-inhibited cell cultures infected with fibroma virus.
J Virol. 1969 Nov;4(5):603-9 PMID: 5391160
Induction of delayed hypersensitivity to influenza and mumps viruses in mice.
J Immunol. 1969 Oct;103(4):844-9 PMID: 5349737
Origin of the lung macrophage. Evidence derived from radiation injury.
Arch Pathol. 1969 Nov;88(5):540-6 PMID: 5347143
In vitro studies on the mechanism of macrophage migration inhibition in tuberculin sensitivity.
J Immunol. 1968 Nov;101(5):1004-15 PMID: 5748594
Studies on the migration inhibitory factor associated with delayed-type hypersensitivity: cytodynamics and specificity.
Transplantation. 1967 Jul;5(4):Suppl:996-1000 PMID: 6049785
Leukocytes and interferon in the host response to viral infections. II. Enhanced interferon response of leukocytes from immune animals.
J Bacteriol. 1966 Jun;91(6):2185-91 PMID: 5943935
Immunologic factors involved in the growth of primary tumors in human or animal hosts.
Cancer Res. 1969 Sep;29(9):1741-6 PMID: 4390408
Studies on pulmonary alveolar macrophages from the normal rabbit: a technique to procure them in a high state of purity.
J Immunol. 1961 Feb;86:128-32 PMID: 13727273
Mechanism of a reaction in vitro associated with delayed-type hypersensitivity.
Science. 1966 Jul 1;153(3731):80-2 PMID: 5938421
Specific role of each human leukocyte type in viral infections. II. Phytohemagglutinin-treated lymphocytes as host cells for vesicular stomatitis virus replication in vitro.
J Virol. 1968 May;2(5):440-8 PMID: 4301314

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.2.6.783-790.1970

Published

1970-12-00

Pages

783-90

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC416092

External Links

PubMed source DOI: 10.1128/iai.2.6.783-790.1970 PMC Full Text View journal details

Analysis Services

In vitro assessment of cellular immunity to vaccinia virus: contribution of lymphocytes and macrophages.

Contact

WeChat Official Account