Characteristics of cytophilic antibody in the mouse.

Infection and immunity ·Vol. 3 ·No. 5 ·1971-05-00 ·Pages 637-41

Brown GL, Carpenter PL

Abstract

Immunoglobulin M hemagglutinin and antibody cytophilic for macrophages were produced simultaneously in mice after primary antigenic stimulation with sheep erythrocytes in saline. Antigen emulsified in Freund's complete adjuvant induced formation of cytophilic antibody (CA) in high titer. Purified cytophilic globulin was isolated by permitting it to attach to macrophages in vitro and eluting it at 56 C. The chromatographic elution pattern, molecular weight, and resistance to mercaptoethanol suggested that CA is a member of the immunoglobulin G class of immunoglobulins.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Brown G L

Microbiology Division, U.S. Army Medical Research and Nutrition Laboratory, Fitzsimons General Hospital, Denver, Colorado 80240.

Carpenter P L

References (11)

11 references, click to expand

The adsorption of antigen by spleen cells previously treated with antiserum in vitro.
Immunology. 1960 Jul;3:272-83 PMID: 13803569
CYTOPHILIC ANTIBODY IN GUINEA-PIGS WITH DELAYED-TYPE HYPERSENSITIVITY.
Immunology. 1964 Jul;7:474-83 PMID: 14193159
THE BASIS FOR IMMUNITY TO MOUSE TYPHOID. 3. CELL-BOUND ANTIBODY.
Aust J Exp Biol Med Sci. 1964 Apr;42:237-48 PMID: 14150758
Properties of antibodies cytophilic for macrophages.
J Exp Med. 1966 Jan 1;123(1):119-44 PMID: 4159249
Passive sensitization of tissue cells. 3. A primate macrophage-cytophilic antibody.
Int Arch Allergy Appl Immunol. 1970;37(2):113-23 PMID: 4983459
Macrophage cytophilic antibodies and delayed hypersensitivity.
Br Med Bull. 1967 Jan;23(1):15-20 PMID: 5342372
Sedimentation properties of antibody cytophilic for macrophages.
J Immunol. 1968 Jun;100(6):1219-22 PMID: 5696284
Experimental delayed type allergy without demonstrable antibodies. I. Absence of cytophilic antibodies.
Immunology. 1967 Jun;12(6):701-12 PMID: 6027424
Cytophilic antibody. I. Nature of the macrophage receptor.
Immunology. 1967 Jan;12(1):13-20 PMID: 6017281
Characterization of a small proteolytic enzyme which lyses bacterial cell walls.
J Bacteriol. 1966 Feb;91(2):524-34 PMID: 5935339
Passive sensitization of tissue cells. 1. Passive sensitization of macrophages by guinea-pig cytophilic antibody.
Int Arch Allergy Appl Immunol. 1965;28(1):87-104 PMID: 5862067

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.3.5.637-641.1971

Published

1971-05-00

Pages

637-41

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC416209

External Links

PubMed source DOI: 10.1128/iai.3.5.637-641.1971 PMC Full Text View journal details

Analysis Services

Characteristics of cytophilic antibody in the mouse.

Contact

WeChat Official Account