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PMID: 16559073 Published · ppublish English Journal Article

Sensitivity and Resistance to Spectinomycin in Escherichia coli.

Journal of bacteriology ·Vol. 100 ·No. 2 ·1969-11-00 ·Pages 939-47

Anderson P

Abstract

Inhibition of growth and division of Escherichia coli by spectinomycin is reversible, and the kinetics of its interference with deoxyribonucleic and ribonucleic acid synthesis may be interpreted as secondary effects of inhibition of protein synthesis on the ribosome. Spontaneous mutations to spectinomycin resistance occur in E. coli K-12 at a rate of about 2 x 10(-10). Resistance is transducible with a discrete lag in phenotypic expression, and the kinetics of its development is about the same as that for streptomycin resistance. All spectinomycin-resistant mutants tested contain resistant ribosomes, and all map in a locus (spc) counterclockwise to and 70% cotransducible with the classical str locus. Differences in the residual drug sensitivity of various spectinomycin-resistant mutants, and of their ribosomes, indicate the existence of more than one phenotypic class of resistance.

Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Anderson P
Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts 02115.
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.100.2.939-947.1969
Published
1969-11-00
Pages
939-47
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC250178
External Links
PubMed source DOI: 10.1128/jb.100.2.939-947.1969 PMC Full Text View journal details
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