CYTOPATHIC EFFECT OF THE ATYPICAL PNEUMONIA ORGANISM IN CULTURES OF HUMAN TISSUE.
Eaton, Monroe D. (Harvard Medical School, Boston, Mass.), Ann E. Farnham, Jeana D. Levinthal, and Anthony R. Scala. Cytopathic effect of the atypical pneumonia organism in cultures of human tissue. J. Bacteriol. 84:1330-1337. 1962.-Three strains of the atypical pneumonia agent were adapted to grow in continuous cell cultures of human amnion or human embryonic lung, with production of initial increased acidity followed by destruction of the cells. Evidence is presented that cytopathic effects of the organism were associated with intracellular growth and formation of microcolonies. Clumps of organisms stained specifically with fluorescein-labeled antibody, and showed distinctive tinctorial reactions with the May Grünwald-Giemsa stain. The cytopathic effect was prevented by fresh serum from a rabbit immunized with an egg-passage strain of the atypical pneumonia agent. Heating the immune serum to 56 C for 30 min abolished the neutralizing effect. The significance of heat-labile serum constituents in killing or inhibition of mycoplasma is discussed.
14 references, click to expand
-
Inhibition of growth of pleuropneumonia-like organisms by antibody.J Pathol Bacteriol. 1954 Jul;68(1):23-30 PMID: 13212552
-
Reversion of a pleuropneumonia-like organism to a Corynebacterium during tissue culture passage.J Gen Microbiol. 1956 Jul;14(3):763-74 PMID: 13346036
-
Visualization and morphology of pleuropneumonialike organisms in clinical material.J Bacteriol. 1957 Feb;73(2):162-71 PMID: 13416165
-
Studies on primary atypical pneumonia. I. Localization, isolation, and cultivation of a virus in chick embryos.J Exp Med. 1957 Oct 1;106(4):455-66 PMID: 13475605
-
Studies on sensitivity to streptomycin of the atypical pneumonia agent.J Bacteriol. 1957 Dec;74(6):784-7 PMID: 13502305
-
Pleuropneumonia-like (mycoplasma) infections of tissue culture.J Bacteriol. 1961 Apr;81:626-35 PMID: 13691094
-
Growth of laboratory and naturally occurring strains of Eaton agent in monkey kidney tissue culture.Proc Soc Exp Biol Med. 1960 Nov;105:371-5 PMID: 13692352
-
Growth of the Eaton agent of primary atypical pneumonia in chick entodermal tissue culture.Proc Soc Exp Biol Med. 1960 Nov;105:375-7 PMID: 13707157
-
Studies on primary atypical pneumonia. III. A factor in normal serum which enhances the reaction between PAP virus and convalescent serum.J Exp Med. 1961 Jan 1;113:111-23 PMID: 13762785
-
Effect of an organic gold salt on Eaton's primary atypical pneumonia agent and other observations.Nature. 1961 Jan 21;189:247-8 PMID: 13767007
-
Fine structure of pleuropneumonia-like organisms in pure culture and in infected tissue culture cells.J Bacteriol. 1960 Feb;79:267-76 PMID: 13819408
-
Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO.Proc Natl Acad Sci U S A. 1962 Jan 15;48:41-9 PMID: 13878126
-
Demonstration of Eaton's agent in tissue culture.Proc Soc Exp Biol Med. 1961 Aug-Sep;107:715-8 PMID: 13880016
-
Intracellular growth of pleuropneumonialike organisms (PPLO) in tissue culture and in ovo.Ann N Y Acad Sci. 1960 Jan 15;79:433-49 PMID: 14400338
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
