ISOLATION AND PROPERTIES OF AN EXOCELLULAR NUCLEASE OF SERRATIA MARCESCENS.

Journal of bacteriology ·Vol. 85 ·No. 2 ·1963-02-00 ·Pages 273-8

Eaves GN, Jeffries CD

Abstract

Eaves, George N. (Wayne State University College of Medicine, Detroit, Mich.) and Charles D. Jeffries. Isolation and properties of an exocellular nuclease of Serratia marcescens. J. Bacteriol. 85:273-278. 1963.-The exocellular nuclease of Serratia marcescens, isolated by anion-exchange chromatography on diethylaminoethyl-Sephadex, depolymerized deoxyribonucleic acid, ribonucleic acid, and the polynucleotide which is refractory to pancreatic ribonuclease activity. The enzyme was tentatively classified as a nonspecific phosphodiesterase. Magnesium was essential for activity, which was optimal at pH 8.8. The purified enzyme was completely inactivated by heating at 50 C for 15 min.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Eaves G N

Department of Microbiology, Wayne State University College of Medicine, Detroit, Michigan.

Jeffries C D

References (9)

9 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.85.2.273-278.1963

Published

1963-02-00

Pages

273-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC278129

External Links

PubMed source DOI: 10.1128/jb.85.2.273-278.1963 PMC Full Text View journal details

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