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PMID: 1656233 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Unregulated expression of the erythropoietin receptor gene caused by insertion of spleen focus-forming virus long terminal repeat in a murine erythroleukemia cell line.

Molecular and cellular biology ·Vol. 11 ·No. 11 ·1991-11-00 ·Pages 5527-33

Hino M, Tojo A, Misawa Y, Morii H, Takaku F, Shibuya M

Abstract

A murine erythroleukemia (MEL) cell line, F5-5, expressed 10,000 binding sites for erythropoietin (EPO) per cell, 10-fold more than was expressed by other murine erythroleukemia cell lines and normal erythroid progenitors. Northern (RNA) and Southern blot analyses revealed overexpression of mRNA for the EPO receptor (EPOR) and rearrangement of one of the EPOR gene alleles in F5-5 cells, respectively. Molecular cloning of F5-5-derived cDNA encoding EPOR revealed that the 5' noncoding region of the EPOR cDNA corresponds to the 3' long terminal repeat sequence of the polycythemic strain of Friend spleen focus-forming virus (F-SFFVP). The aberrant EPOR transcripts containing the 3' long terminal repeat sequence were mainly expressed in F5-5 cells. The same integration upstream of the EPOR gene was also observed in other subclones and the parent cell line. It is possible that overexpression of EPOR by viral promoter insertion will confer growth advantage to an F-SFFVP-infected erythroid progenitor cell, leading to positive clonal selection through further leukemogenic steps.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular DNA, Neoplasm/genetics Erythropoietin/metabolism Gene Expression Regulation, Viral Kinetics Leukemia, Erythroblastic, Acute Mice Molecular Sequence Data Mutagenesis, Insertional Oligonucleotides Polymerase Chain Reaction Receptors, Cell Surface/genetics,metabolism Receptors, Erythropoietin Repetitive Sequences, Nucleic Acid Restriction Mapping Spleen Focus-Forming Viruses/genetics
Chemicals
DNA, Neoplasm Oligonucleotides Receptors, Cell Surface Receptors, Erythropoietin Erythropoietin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hino M
Department of Genetics, University of Tokyo, Japan.
Tojo A
Misawa Y
Morii H
Takaku F
Shibuya M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.11.5527-5533.1991
Published
1991-11-00
Pages
5527-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361922
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.11.5527-5533.1991 PMC Full Text View journal details
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