Home LiteratureArticle Details

PMID: 165902 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Differential cytotoxic acitivity of anticollagen serum on rat osteoblasts and fibroblasts in tissue culture.

Cell ·Vol. 5 ·No. 1 ·1975-05-00 ·Pages 83-6

Duksin D, Maoz A, Fuchs S

Abstract

Rat skin fibroblasts grown in tissue culture were lysed by anti-rat-tail collagen serum and antibodies to the ordered collagen-like synthetic polymer (Pro-Gly-Pro)-n. This cytotoxic effect is complement-dependent and occurs only if the fibroblasts were pretreated with trypsin. These anti-sera have very little cytotoxicity on cultured rat osteoblasts. This differential cytotoxicity is not due to differential binding of anticollagen serum to the cells. Both osteoblasts and skin fibroblasts bind the anticollagen serum as was demonstrated by fluorescent immunoglobulin.

MeSH Terms

Animals Antibodies Cell Line Cell Transformation, Neoplastic Collagen/immunology Culture Techniques Cytotoxicity Tests, Immunologic Fibroblasts/immunology Fluorescent Antibody Technique Immune Sera L-Lactate Dehydrogenase/blood Lung Neoplasms Neoplasms, Experimental Osteoblasts/immunology Peptides/immunology Polyomavirus Rabbits/immunology Rats Skin Trypsin/pharmacology

Chemicals

Antibodies Immune Sera Peptides Collagen L-Lactate Dehydrogenase Trypsin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Duksin D

Maoz A

Fuchs S

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/0092-8674(75)90095-1

Published

1975-05-00

Pages

83-6

Language

English

Region

United States

NLM ID

0413066

Subset

External Links

PubMed source DOI: 10.1016/0092-8674(75)90095-1 View journal details

Analysis Services

Differential cytotoxic acitivity of anticollagen serum on rat osteoblasts and fibroblasts in tissue culture.

Contact

WeChat Official Account