A 5'-Adenosine Monophosphate-Dependent Adenylate Cyclase and an Adenosine 3':5'-Cyclic Monophosphate-Dependent Adenosine Triphosphate Pyrophosphohydrolase in Dictyostelium discoideum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 4 ·1973-04-00 ·Pages 1254-7

Rossomando EF, Sussman M

Abstract

Cell aggregation in Dictyostelium discoideum appears to involve the production and detection of 3':5'-cyclic AMP. Two pertinent catalytic activities have been studied in concentrates from D. discoideum purified 50- to 100-fold. They are (i) adenylate cyclase and (ii) ATP pyrophosphohydrolase. ATP pyrophosphohydrolase activity converts ATP to 5'-AMP and pyrophosphate. The presence of 5'-AMP is an absolute requirement for adenylate cyclase activity. The presence of 3':5'-cyclic AMP is an absolute requirement for ATP pyrophosphohydrolase activity. Both activations, particularly that of ATP pyrophosphohydrolase, show narrow ranges of specificity and display significant cooperativities.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Rossomando E F

Department of Biology, Brandeis University, Waltham, Massachusetts 02154.

Sussman M

References (12)

12 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.4.1254

Published

1973-04-00

Pages

1254-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433470

External Links

PubMed source DOI: 10.1073/pnas.70.4.1254 PMC Full Text View journal details

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A 5'-Adenosine Monophosphate-Dependent Adenylate Cyclase and an Adenosine 3':5'-Cyclic Monophosphate-Dependent Adenosine Triphosphate Pyrophosphohydrolase in Dictyostelium discoideum.

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