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PMID: 16592735 Published · ppublish English Journal Article

Isolation and structure of pro-methionine-enkephalin: Potential enkephalin precursor from porcine hypothalamus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 12 ·1979-12-00 ·Pages 6177-80

Huang WY, Chang RC, Kastin AJ, Coy DH, Schally AV

Abstract

A hexapeptide that we have named pro-methionine-enkephalin has been isolated from acid extracts of porcine hypothalami and found to have the amino acid sequence H-Tyr-Gly-Gly-Phe-Met(O)-Arg-OH. This peptide is not a fragment of either porcine beta-lipotropin or beta-endorphin, which suggests that it could be a precursor of [Met]enkephalin in the brain by a pathway differing from the one usually postulated. The existence of still larger precursors of [Met]enkephalin, perhaps related to the recently reported alpha-neo-endorphin, is strongly implied by these studies. Synthetic H-Tyr-Gly-Gly-Phe-Met-Arg-OH exhibited low, but significant, opiate activity in vitro.

Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Huang W Y
Endocrine and Polypeptide Laboratory, Veterans Administration Medical Center, New Orleans, Louisiana 70146.
Chang R C
Kastin A J
Coy D H
Schally A V
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.12.6177
Published
1979-12-00
Pages
6177-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411826
External Links
PubMed source DOI: 10.1073/pnas.76.12.6177 PMC Full Text View journal details
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