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PMID: 16593250 Published · ppublish English Journal Article

Location and nucleotide sequence of the gene for the proton-translocating subunit of wheat chloroplast ATP synthase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 22 ·1982-11-00 ·Pages 6903-7

Howe CJ, Auffret AD, Doherty A, Bowman CM, Dyer TA, Gray JC

Abstract

The proton-translocating subunit of wheat chloroplast ATP synthase is encoded by a chloroplast gene that has been accurately mapped and whose nucleotide sequence has been determined. The predicted sequence of 81 amino acids has been confirmed in part by determination of the sequence of the first 40 amino acids from the NH(2) terminus of the protein, and it shows 100% homology with the known amino acid sequence of the spinach protein but no more than 35% homology with the amino acid sequences of bacterial and mitochondrial proteins. The gene shows no deviation from the "universal" genetic code and is not split. A potential ribosome binding site is located 12 nucleotides upstream from the initiation codon, but sequences homologous to prokaryotic promotors and transcription terminators are not apparent.

Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Howe C J
Botany School, University of Cambridge, Downing Street, Cambridge CB2 3EA. United Kingdom.
Auffret A D
Doherty A
Bowman C M
Dyer T A
Gray J C
References (28)
28 references, click to expand
  1. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  2. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  3. Nucleotide sequence of genes coding for dicyclohexylcarbodiimide-binding protein and the alpha subunit of proton-translocating ATPase of Escherichia coli.
    Biochem Biophys Res Commun. 1981 May 15;100(1):219-25 PMID: 6266400
  4. The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA.
    Nucleic Acids Res. 1981 Jul 24;9(14):3251-70 PMID: 6269077
  5. The dicyclohexylcarbodiimide-binding protein c of ATP synthase from Escherichia coli is not sufficient to express an efficient H+ conduction.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6643-6 PMID: 6273880
  6. Small is beautiful--portrait of a mitochondrial genome.
    Nature. 1981 Apr 9;290(5806):443-4 PMID: 7219533
  7. The atp operon: nucleotide sequence of the promoter and the genes for the membrane proteins, and the delta subunit of Escherichia coli ATP-synthase.
    Nucleic Acids Res. 1981 Aug 25;9(16):3919-26 PMID: 6272190
  8. Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase.
    Nature. 1981 May 14;291(5811):117-21 PMID: 7015143
  9. Sequence of a putative promoter region for the rRNA genes of tobacco chloroplast DNA.
    Nucleic Acids Res. 1981 Oct 24;9(20):5399-406 PMID: 7029469
  10. The complete nucleotide sequence of 16S ribosomal RNA gene from tobacco chloroplasts.
    Gene. 1982 Feb;17(2):213-8 PMID: 7044901
  11. Processing of a chloroplast-translated membrane protein in vivo. Analysis of the rapidly synthesized 32 000-dalton shield protein and its precursor in Spirodela oligorrhiza.
    Eur J Biochem. 1982 May;124(1):125-9 PMID: 7084221
  12. Use of fluorescamine as an effective blocking reagent to reduce the background in protein sequence analyses by the Beckman automated sequencer.
    Anal Biochem. 1981 Mar 15;112(1):158-62 PMID: 7258621
  13. Synthesis of a dicyclohexylcarbodiimide-binding proteolipid by isolated pea chloroplasts.
    Eur J Biochem. 1980;108(1):131-6 PMID: 7408844
  14. Rearrangements in the chloroplast genomes of mung bean and pea.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5533-7 PMID: 16593087
  15. Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2375-8 PMID: 18736
  16. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  17. Mu-induced polarity in the unc operon of Escherichia coli.
    J Bacteriol. 1978 Jun;134(3):728-36 PMID: 149112
  18. The dicyclohexylcarbodiimide-binding protein of the mitochondrial ATPase complex from Neurospora crassa and Saccharomyces cerevisiae. Identification and isolation.
    Eur J Biochem. 1979 Feb 1;93(3):587-99 PMID: 154405
  19. A fifth gene (uncE) in the operon concerned with oxidative phosphorylation in Escherichia coli.
    J Bacteriol. 1979 Feb;137(2):711-8 PMID: 154509
  20. Nucleotide sequence of the mitochondrial structural gene for subunit 9 of yeast ATPase complex.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1663-7 PMID: 156363
  21. Cell-free transcription and translation of total spinach chloroplast DNA.
    Eur J Biochem. 1979 Jan 2;93(1):31-9 PMID: 374074
  22. Polyquarternary amines prevent peptide loss from sequenators.
    Anal Biochem. 1978 Feb;84(2):622-7?0=ENG PMID: 626405
  23. Membrane proteins synthesized but not processed by isolated maize chloroplasts.
    J Cell Biol. 1978 Sep;78(3):734-46 PMID: 701358
  24. Rapid analysis of amino acid phenylthiohydantoins by high-performance liquid chromatography.
    Anal Biochem. 1977 Feb;77(2):569-73 PMID: 842843
  25. Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2175-9 PMID: 1056023
  26. The application of 0.1 M quadrol to the microsequence of proteins and the sequence of tryptic peptides.
    Biochemistry. 1975 Jul;14(13):3029-35 PMID: 1148190
  27. Assembly of the mitochondrial system. Purification of a mitochondrial product of the ATPase.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3155-9 PMID: 4274131
  28. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.
    Anal Biochem. 1981 Apr;112(2):295-8 PMID: 6266279
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.22.6903
Published
1982-11-00
Pages
6903-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347242
External Links
PubMed source DOI: 10.1073/pnas.79.22.6903 PMC Full Text View journal details
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