Methanophosphagen: Unique cyclic pyrophosphate isolated from Methanobacterium thermoautotrophicum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 17 ·1983-09-00 ·Pages 5217-21

Kanodia S, Roberts MF

Abstract

A unique cyclic pyrophosphate compound has been detected at 10-12 mM intracellular concentration in Methanobacterium thermoautotrophicum by in vivo(31)P NMR. This compound has been extracted from cells and purified by anion-exchange chromatography. Studies with (1)H, (13)C, and (31)P NMR and fast-atom-bombardment mass spectrometry have identified it as 2,3-cyclopyrophosphoglycerate, an intramolecularly cyclized pyrophosphate of 2,3-diphosphoglycerate. Chemical degradation to 2,3-diphosphoglycerate and synthesis by dicyclohexylcarbodiimide coupling of 2,3-diphosphoglycerate are consistent with this identification. It is suggested that this compound serves as a primary phosphagen in methanogens.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kanodia S

Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.

Roberts M F

References (13)

13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.80.17.5217

Published

1983-09-00

Pages

5217-21

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC384223

External Links

PubMed source DOI: 10.1073/pnas.80.17.5217 PMC Full Text View journal details

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Methanophosphagen: Unique cyclic pyrophosphate isolated from Methanobacterium thermoautotrophicum.

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