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PMID: 16593382 Published · ppublish English Journal Article

Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 20 ·1983-10-00 ·Pages 6293-7

Spreitzer RJ, Ogren WL

Abstract

Based on the unique ability of chloroplast genes to recombine in Chlamydomonas reinhardtii, a collection of acetate-requiring mutants was screened for recombination with a mutation affecting ribulose-1,5-bisphosphate carboxylase/oxygenase [Rbu-1,5-P(2) carboxylase/oxygenase; 3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39]. This chloroplast mutation, rcl-u-1-10-6C, causes the absence of Rbu-1,5-P(2) carboxylase/oxygenase activities and alters the isoelectric point of the larger subunit. Several mutants that displayed little or no recombination with 10-6C were recovered, and two lacked carboxylase activity. These new chloroplast mutants lack both large and small Rbu-1,5-P(2) carboxylase/oxygenase subunits. The approach demonstrated here permits the routine recovery of chloroplast mutations affecting this enzyme. Multiple mutations in the Rbu-1,5-P(2) carboxylase/oxygenase large-subunit gene can be used to investigate the function and regulation of this enzyme and the regulation of chloroplast genes in general.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Spreitzer R J
Department of Agronomy, University of Illinois, Urbana, IL 61801.
Ogren W L
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.20.6293
Published
1983-10-00
Pages
6293-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394283
External Links
PubMed source DOI: 10.1073/pnas.80.20.6293 PMC Full Text View journal details
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