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PMID: 16593484 Published · ppublish English Journal Article

Isolation and characterization of a gene for a major light-harvesting polypeptide from Cyanophora paradoxa.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 13 ·1984-07-00 ·Pages 4100-4

Lemaux PG, Grossman A

Abstract

Antibodies raised against mixtures of phycobilisome polypeptides from the eukaryotic alga Cyanidium caldarium were used in an immunological screen to detect expression of phycobiliprotein genes in an Escherichia coli library containing segments of plastid (chloroplast, cyanelle) DNA from another eukaryotic alga, Cyanophora paradoxa. The four candidate clones obtained were mapped by restriction analysis and found to be overlapping. The clone with the smallest insert (1.4 kilobases) was partially sequenced and a coding region similar to the carboxyl terminus of the phycobiliprotein subunit beta-phycocyanin was found. The coding region for the beta-phycocyanin gene in C. paradoxa has been mapped to the small single copy region on the cyanelle genome, and its orientation has been determined. A short probe unique to a conserved chromophore binding site shared by at least two phycobiliprotein subunits has now been generated from the carboxyl terminus of the beta-phycocyanin gene. This probe may be useful in identifying specific phycobiliprotein subunit genes, beta-phycocyanin, beta-phycoerythrocyanin, and possibly beta-phycoerythrin, in other eukaryotic algae and in prokaryotic cyanobacteria.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lemaux P G
Department of Plant Biology, Carnegie Institution of Washington, 290 Panama Street, Stanford, CA 94305.
Grossman A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.13.4100
Published
1984-07-00
Pages
4100-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345376
External Links
PubMed source DOI: 10.1073/pnas.81.13.4100 PMC Full Text View journal details
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