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PMID: 16593543 Published · ppublish English Journal Article

Nitrogenase reductase: A functional multigene family in Rhizobium phaseoli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 4 ·1985-02-00 ·Pages 1170-4

Quinto C, De La Vega H, Flores M, Leemans J, Cevallos MA, Pardo MA, Azpiroz R, De Lourdes Girard M, Calva E, Palacios R

Abstract

The complete coding sequence of the nitrogenase reductase gene (nifH) is present in three different regions of a Rhizobium phaseoli symbiotic plasmid. Homology between two of the regions containing nifH coding sequences extends over 5 kilobases. These in turn share 1.3 kilobases of homology with the third region. The nucleotide sequences of the three nitrogenase reductase genes were found to be identical. Site-directed insertion mutagenesis indicated that none of the three genes is indispensable for nitrogen fixation during symbiosis with Phaseolus vulgaris. This implies that at least two of the reiterated genes can be functionally expressed.

Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Quinto C
Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Apartado postal 565-A, Cuernavaca, Morelos, Mexico.
De La Vega H
Flores M
Leemans J
Cevallos M A
Pardo M A
Azpiroz R
De Lourdes Girard M
Calva E
Palacios R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.4.1170
Published
1985-02-00
Pages
1170-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397216
External Links
PubMed source DOI: 10.1073/pnas.82.4.1170 PMC Full Text View journal details
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