Cloning of genes developmentally regulated during plant embryogenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 7 ·1987-04-00 ·Pages 1906-10

Choi JH, Liu LS, Borkird C, Sung ZR

Abstract

Genes specifically induced during somatic embryogenesis may play key roles in plant embryo development. An antiserum against an extract of carrot somatic embryos revealed a few rare antigens induced at the onset of embryogenesis. Through differential immunoadsorption techniques, we purified antibodies against the embryo-specific antigens and probed a phage lambda gt11 library of cDNA from carrot somatic embryos. This paper describes three distinct cDNA clones that hybridize to embryo-specific RNAs. Monospecific antibodies, purified by affinity to the recombinant phage fusion proteins, confirm that the cloned cDNAs encode unique embryo-specific peptide antigens. One 50-kDa protein correlates with embryogenic ability in cultures of other plant species, including cereals.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Choi J H

Department of Genetics, University of California, Berkeley, CA 94720.

Liu L S

Borkird C

Sung Z R

References (15)

15 references, click to expand

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
Turbidimetric measurement of plant cell culture growth.
Plant Physiol. 1976 Mar;57(3):460-2 PMID: 16659505
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Developmental regulation of cloned superabundant embryo mRNAs in soybean.
Dev Biol. 1981 Apr 30;83(2):218-31 PMID: 6113180
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
Anal Biochem. 1981 Nov 1;117(2):307-10 PMID: 6172996
India ink staining of proteins on nitrocellulose paper.
Anal Biochem. 1983 Aug;133(1):157-62 PMID: 6195938
Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots.
J Cell Biol. 1984 Jul;99(1 Pt 1):20-8 PMID: 6203917
Efficient isolation of genes by using antibody probes.
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
Gene. 1983 Dec;26(1):101-6 PMID: 6323249
Identification of concanavalin A-binding proteins after sodium dodecyl sulfate--gel electrophoresis and protein blotting.
Anal Biochem. 1982 Jun;123(1):143-6 PMID: 7114467
Use of a cloned library for the study of abundant poly(A)+RNA during Xenopus laevis development.
Dev Biol. 1980 May;76(2):449-64 PMID: 7390013
Embryonic proteins in somatic embryos of carrot.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3683-7 PMID: 16593035
Coordinate gene expression during somatic embryogenesis in carrots.
Proc Natl Acad Sci U S A. 1983 May;80(9):2661-5 PMID: 16593311
Inhibition of gene expression in plant cells by expression of antisense RNA.
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5372-6 PMID: 16593734
Identification of human glucocorticoid receptor complementary DNA clones by epitope selection.
Science. 1985 May 10;228(4700):740-2 PMID: 2581314

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.84.7.1906

Published

1987-04-00

Pages

1906-10

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC304550

External Links

PubMed source DOI: 10.1073/pnas.84.7.1906 PMC Full Text View journal details

Analysis Services

Cloning of genes developmentally regulated during plant embryogenesis.

Contact

WeChat Official Account