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PMID: 16603664 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nitric oxide- and hydrogen peroxide-responsive gene regulation during cell death induction in tobacco.

Plant physiology ·Vol. 141 ·No. 2 ·2006-06-00 ·Pages 404-11

Zago E, Morsa S, Dat JF, Alard P, Ferrarini A, Inzé D, Delledonne M, Van Breusegem F

Abstract

Nitric oxide (NO) and hydrogen peroxide (H(2)O(2)) are regulatory molecules in various developmental processes and stress responses. Tobacco (Nicotiana tabacum) leaves exposed to moderate high light dramatically potentiated NO-mediated cell death in catalase-deficient (CAT1AS) but not in wild-type plants, providing genetic evidence for a partnership between NO and H(2)O(2) during the induction of programmed cell death. With this experimental model system, the specific impact on gene expression was characterized by either NO or H(2)O(2) alone or both molecules combined. By means of genome-wide cDNA-amplified fragment length polymorphism analysis, transcriptional changes were compared in high light-treated CAT1AS and wild-type leaves treated with or without the NO donor sodium nitroprusside. Differential gene expression was detected for 214 of the approximately 8,000 transcript fragments examined. For 108 fragments, sequence analysis revealed homology to genes with a role in signal transduction, defense response, hormone interplay, proteolysis, transport, and metabolism. Surprisingly, only 16 genes were specifically induced by the combined action of NO and H(2)O(2), whereas the majority were regulated by either of them alone. At least seven transcription factors were mutually up-regulated, indicating significant overlap between NO and H(2)O(2) signaling pathways. These results consolidate significant cross-talk between NO and H(2)O(2), provide new insight into the early transcriptional response of plants to increased NO and H(2)O(2) levels, and identify target genes of the combined action of NO and H(2)O(2) during the induction of plant cell death.

MeSH Terms
Base Sequence Cell Death DNA Primers DNA, Complementary Gene Expression Profiling Gene Expression Regulation, Plant Hydrogen Peroxide/metabolism Molecular Sequence Data Nitric Oxide/metabolism Plant Leaves/cytology,metabolism Reverse Transcriptase Polymerase Chain Reaction Tobacco/cytology,genetics,metabolism
Chemicals
DNA Primers DNA, Complementary Nitric Oxide Hydrogen Peroxide
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Zago Elisa
Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, B-9052 Ghent, Belgium.
Morsa Stijn
Dat James F
Alard Philippe
Ferrarini Alberto
Inzé Dirk
Delledonne Massimo
Van Breusegem Frank
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.106.078444
Published
2006-06-00
Epub
2006-00-07
Pages
404-11
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1475440
Subset
IM
Databases
GENBANK
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External Links
PubMed source DOI: 10.1104/pp.106.078444 PMC Full Text View journal details
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