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PMID: 1663292 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genomic sequences homologous to the protein kinase region of the bifunctional herpes simplex virus type 2 protein ICP10.

Virus genes ·Vol. 5 ·No. 3 ·1991-07-00 ·Pages 215-26

Smith CC, Wymer JP, Luo J, Aurelian L

Abstract

The large subunit of herpes simplex virus type 2 (HSV-2) ribonucleotide reductase (ICP10) consists of two functional domains. The amino (N)-terminal domain at residues 1-411 has serine/threonine-specific kinase activity (PK domain) and is encoded by a DNA fragment with transforming potential (15,17). The remaining region is required for ribonucleotide reductase activity (RR domain) (14, 15). Computer-assisted comparison of the ICP10 sequence to the EMBL database 21 has revealed sequences within the RR domain that are common to all RR1 proteins. Motifs homologous to the catalytic domains of all PKs were identified in the PK region (15). However, based on this database all other sequences were unique. Secondary structure analysis of the PK and RR junction region of ICP10 identified twist angle variations with helical periodicity characteristic of enhancer elements. Sequences homologous to a segment of the PK domain were amplified and cloned from human DNA using the polymerase chain reaction (PCR), suggesting that the PK domain may have originated from a cellular gene.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Computers Electrophoresis, Polyacrylamide Gel Female Genes, Viral Humans Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction Protein Kinases/genetics Ribonucleotide Reductases/genetics Sequence Homology, Nucleic Acid Simplexvirus/genetics Uterine Cervical Dysplasia/genetics,microbiology Viral Proteins/genetics
Chemicals
Oligonucleotide Probes Viral Proteins Ribonucleotide Reductases Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smith C C
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201.
Wymer J P
Luo J
Aurelian L
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Article Info
Journal
Virus genes
Abbr.
Virus Genes
ISSN
0920-8569
DOI
10.1007/BF00568971
Published
1991-07-00
Pages
215-26
Language
English
Region
United States
NLM ID
8803967
Subset
IM
Grants
NCI NIH HHS · CA-39691 · United States
External Links
PubMed source DOI: 10.1007/BF00568971 View journal details
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