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PMID: 166377 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Properties of cytoplasmic mutants of Saccharomyces cerevisiae with specific lesions in cytochrome oxidase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2054-7

Tzagoloff A, Akai A, Needleman RB

Abstract

Two mutants with specific defects in cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase; EC 1.9.3.1) have been isolated from cultures of Saccharomyces cerevisiae exposed to the mutagens ethyl-methane sulfonate and Mn++. The mutations have been shown to be extranuclear by two criteria. The phenotype persists in diploids formed by a cross with a p-o strain of yeast of the opposite mating type. Tetrad analysis indicates a non-Mendelian segregation (4:0 and 0:4) of the mutations. Both mutants show a total absence of cytochrome oxidase activity and of spectral cytochromes a and as. One of the mutants has been shown to be missing a polypeptide synthesized by mitochondria. The migration of this protein on polyacrylamide gels corresponds to the highest-molecular-weight subunit of cytochrome oxidase.

MeSH Terms
Diploidy Electron Transport Complex IV/biosynthesis Electrophoresis, Polyacrylamide Gel Ethyl Methanesulfonate/pharmacology Extrachromosomal Inheritance Genetic Complementation Test Genetics, Microbial Macromolecular Substances Manganese/pharmacology Mitochondria/analysis,enzymology Mutation Peptides/analysis Saccharomyces cerevisiae/analysis,drug effects,enzymology,ultrastructure
Chemicals
Macromolecular Substances Peptides Manganese Ethyl Methanesulfonate Electron Transport Complex IV
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tzagoloff A
Akai A
Needleman R B
References (15)
15 references, click to expand
  1. The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide.
    J Mol Biol. 1970 Sep 14;52(2):323-35 PMID: 5485912
  2. Mitochondrial genetics. I. Methodology and phenomenology.
    Symp Soc Exp Biol. 1970;24:449-96 PMID: 5516343
  3. [The adaptative synthesis of cytochromes in baker's yeast].
    Biochim Biophys Acta. 1950 Nov;6(2):256-67 PMID: 14800991
  4. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  5. The mutational alteration of the primary structure of yeast iso-1-cytochrome c.
    J Biol Chem. 1968 Oct 25;243(20):5446-56 PMID: 4178406
  6. Assembly of the mitochondrial membrane system. IV. Role of mitochondrial and cytoplasmic protein synthesis in the biosynthesis of the rutamycin-sensitive adenosine triphosphatase.
    J Biol Chem. 1971 May 10;246(9):3050-6 PMID: 4251997
  7. Assembly of the mitochondrial membrane system. I. Characterization of some enzymes of the inner membrane of yeast mitochondria.
    J Biol Chem. 1969 Sep 25;244(18):5020-6 PMID: 4309989
  8. Cycloheximide resistant incorporation of amino acids into a polypeptide of the cytochrome oxidase of Neurospora crassa.
    Eur J Biochem. 1971 Sep 13;22(1):19-26 PMID: 4329217
  9. Inhibition of the assembly of cytochrome oxidase in Neurospora crassa by chloramphenicol.
    Eur J Biochem. 1972 Nov 7;30(3):413-7 PMID: 4344826
  10. Cytochrome c oxidase from bakers' yeast. II. Site of translation of the protein components.
    J Biol Chem. 1973 Feb 25;248(4):1355-60 PMID: 4346953
  11. Assembly of the mitochondrial membrane system. X. Mitochondrial synthesis of three of the subunit proteins of yeast cytochrome oxidase.
    J Biol Chem. 1973 Jun 25;248(12):4275-9 PMID: 4351219
  12. A novel respiration-deficient mutant of Saccharomyces cerevisiae. I. Preliminary characterization of phenotype and mitochondrial inheritance.
    J Biol Chem. 1974 Oct 10;249(19):6130-7 PMID: 4371758
  13. Studies on energy-linked reactions. Genetic analysis of oligomycin-resistant mutants of Saccharomyces cerevisiae.
    Eur J Biochem. 1973 Jan 15;32(2):312-21 PMID: 4569078
  14. Induction by manganese of mitochondrial antibiotic resistance mutations in yeast.
    Mol Gen Genet. 1973 Nov 22;126(4):357-66 PMID: 4593998
  15. The biogenesis of mitochondria, VI. Biochemical basis of the resistance of Saccharomyces cerevisiae toward antibiotics which specifically inhibit mitochondrial protein synthesis.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1288-93 PMID: 5240029
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2054
Published
1975-06-00
Pages
2054-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432691
Subset
IM
Grants
NHLBI NIH HHS · R01 HL022174 · United States
External Links
PubMed source DOI: 10.1073/pnas.72.6.2054 PMC Full Text View journal details
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